Gene Expression Data
Assay Details
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Assay
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Reference:
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J:176607
Kameda Y, et al., Hes1 regulates the number and anterior-posterior patterning of mesencephalic dopaminergic neurons at the mid/hindbrain boundary (isthmus). Dev Biol. 2011 Oct 1;358(1):91-101
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6280486
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Gene symbol:
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Pitx3
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Gene name:
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paired-like homeodomain transcription factor 3
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Antibody:
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Pitx3 antibody
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
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Results
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Specimen
3D:
embryonic day 12.5
(more  )
Specimen
3D:
(close  )
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Genetic Background:
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involves: CD-1
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: midbrain |
Present |
Regionally restricted |
3D
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Expression in mesencephalic dopaminergic (mesDa) neurons that are distributed perpendicularly. Expression restricted to nuclei of MesDA neurons.
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Specimen
3I:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * CD-1
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Mutant Allele(s):
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Hes1tm1Fgu/Hes1tm1Fgu
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: midbrain |
Present |
Regionally restricted |
3I
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Authors state mesencephalic dopaminergic (mesDa) neurons are extended transversely. Expression in neurons widely distributed in midline area of the thinned midbrain.
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Specimen
5C:
embryonic day 13.5
(more )
Specimen
5C:
(close )
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Genetic Background:
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involves: CD-1
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: midbrain |
Strong |
Regionally restricted |
5C
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Intense expression in mesencephalic dopaminergic (mesDA) neurons. Expression in ventral tegmental area, oriented perpendicular to ventricular surface, well-developed in the midline.
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Specimen
5F:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * CD-1
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Mutant Allele(s):
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Hes1tm1Fgu/Hes1tm1Fgu
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: midbrain |
Present |
Regionally restricted |
5F
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Authors state mesencephalic dopaminergic (mesDA) neurons are distributed sparsely at the brain surface. Expression in neurons with short perpendicular extensions.
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Specimen
6B:
embryonic day 15.5
(more )
Specimen
6B:
(close )
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Genetic Background:
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involves: CD-1
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: midbrain |
Present |
Regionally restricted |
6B
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Expression in neurons condensed in the ventral midbrain. Authors report expression in substantia nigra compacta (SNc) and ventral tegmental area (VTA) similar to that of mature animals.
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Specimen
6E:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * CD-1
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Mutant Allele(s):
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Hes1tm1Fgu/Hes1tm1Fgu
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: midbrain |
Weak |
Regionally restricted |
6E
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Expression in neurons that are sparsely and widely distributed at the brain surface.
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Specimen
7G:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * CD-1
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Mutant Allele(s):
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Hes1tm1Fgu/Hes1tm1Fgu
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: midbrain |
Present |
Regionally restricted |
7G
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Expression localized mesencephalic dopaminergic (mesDA) neurons.
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Specimen
9E:
embryonic day 13.5; Hes1tm1Fgu/Hes1 +
(more )
Specimen
9E:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * CD-1
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Mutant Allele(s):
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Hes1tm1Fgu/Hes1+
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: hindbrain |
Present |
Regionally restricted |
9E
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Expression in neurons invading the hindbrain. Expression in many neurons distributed in the rostral hindbrain.
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Specimen
10F:
embryonic day 14.5
(more )
Specimen
10F:
(close )
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Genetic Background:
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involves: CD-1
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Specimen
10I:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * CD-1
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Mutant Allele(s):
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Hes1tm1Fgu/Hes1tm1Fgu
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Analysis Tools