Probe:	Porcn probe1
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase
Assay notes:	For in situ hybridization of Porcn, two probes (MGI:6275042 and MGI:6275043) were used simultaneously.

Structure	Level	Pattern	Image	Note
TS9: embryo ectoderm	Present	Regionally restricted	1A	Expression in epiblast cells undergoing gastrulation.
TS9: primitive streak	Present		1A	Expression in epiblast cells forming the primitive streak on the posterior side of the embryo
TS9: anterior visceral endoderm	Present		1A

Structure	Level	Pattern	Image	Note
TS11: embryo	Present	Regionally restricted	1B
TS11: anterior visceral endoderm	Absent		1B

Structure	Level	Pattern	Image	Note
TS13: embryo	Present		1C	Expression restricted to embryo proper.
TS13: cardiogenic plate	Absent		1C
TS13: foregut diverticulum	Absent		1C

Structure	Level	Pattern	Image	Note
TS14: embryo	Strong	Regionally restricted	1D	Strong expression on dorsal side of embryo.
TS14: neural tube	Strong		1D
TS14: head	Moderate	Widespread	1D
TS14: optic vesicle	Moderate		1D
TS14: primitive ventricle	Absent		1D

Structure	Level	Pattern	Image	Note
TS11: embryo endoderm	Strong	Regionally restricted	1E	Higher levels of expression in lateral proximal region of endoderm surrounding the egg cylinder.
TS11: embryo ectoderm	Strong	Regionally restricted	1E	Higher levels of expression in lateral regions of epiblast
TS11: primitive streak	Moderate		1E
TS11: embryo mesoderm	Moderate		1E	Moderate levels of expression in migrating mesoderm.
TS11: embryo	Moderate	Regionally restricted	1E

Structure	Level	Pattern	Image	Note
TS14: eye surface ectoderm	Absent		1F

Structure	Level	Pattern	Image	Note
TS14: embryo	Present	Graded	1G	Gradient of expression with highest levels of dorsal side.
TS14: primitive ventricle	Absent		1G