Gene Expression Data
Assay Details
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Assay
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Reference:
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J:154906
Wang W, et al., Atf4 regulates chondrocyte proliferation and differentiation during endochondral ossification by activating Ihh transcription. Development. 2009 Dec;136(24):4143-53
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:6273224
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Gene symbol:
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Col10a1
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Gene name:
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collagen, type X, alpha 1
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Results
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Specimen
5Am:
embryonic day 14.5
(more  )
Specimen
5Am:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 14.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
5Am':
(close )
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Genetic Background:
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involves: 129
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Mutant Allele(s):
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Atf4tm1Tow/Atf4tm1Tow
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Age:
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embryonic day 14.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: humerus cartilage condensation |
Present |
Regionally restricted |
5Am'
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Authors state expression level was unchanged in Atf4-/- growth plates. Expression in hypertrophic chondrocytes separated into single mass at center of Atf4-/- humerus.
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Specimen
5An:
embryonic day 16.5
(more )
Specimen
5An:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 16.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: humerus |
Present |
Regionally restricted |
5An
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Specimen
5An':
(close )
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Genetic Background:
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involves: 129
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Mutant Allele(s):
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Atf4tm1Tow/Atf4tm1Tow
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Age:
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embryonic day 16.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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Not Specified
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: humerus |
Present |
Regionally restricted |
5An'
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Expression level was unchanged in Atf4-/- growth plates. Zone of expression in hypertrophic chondrocytes slightly expanded compared with wildtype.
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Specimen
5Ao:
postnatal day 0
(more )
Specimen
5Ao:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal day 0
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Sex:
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Not Specified
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Type:
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Not Specified
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: humerus |
Present |
Regionally restricted |
5Ao
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Specimen
5Ao':
(close )
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Genetic Background:
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involves: 129
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Mutant Allele(s):
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Atf4tm1Tow/Atf4tm1Tow
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Age:
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postnatal day 0
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Sex:
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Not Specified
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Type:
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Not Specified
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: humerus |
Present |
Regionally restricted |
5Ao'
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Expression level was unchanged in Atf4-/- growth plates. Authors state that the zone of expression in hypertrophic chondrocytes remained unchanged, or were expanded, if one takes into account the overall length of humeri.
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Analysis Tools