Gene Expression Data
Assay Details
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Assay
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Reference:
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J:154906
Wang W, et al., Atf4 regulates chondrocyte proliferation and differentiation during endochondral ossification by activating Ihh transcription. Development. 2009 Dec;136(24):4143-53
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:6273220
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Gene symbol:
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Ihh
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Gene name:
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Indian hedgehog
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Probe:
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Ihh cDNA8
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Probe preparation:
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Antisense labelled with S35 RNA
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Results
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Specimen
5Aa:
embryonic day 14.5
(more  )
Specimen
5Aa:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 14.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
5Aa':
(close )
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Genetic Background:
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involves: 129
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Mutant Allele(s):
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Atf4tm1Tow/Atf4tm1Tow
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Age:
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embryonic day 14.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
5Ab:
embryonic day 16.5
(more )
Specimen
5Ab:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 16.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: humerus |
Present |
Regionally restricted |
5Ab
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Expression in prehypertrophic chondrocytes increased at E16 and P0 in wildtype humeri. At E16, low level of expression in osteoblasts at center of long bones.
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Specimen
5Ab':
(close )
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Genetic Background:
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involves: 129
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Mutant Allele(s):
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Atf4tm1Tow/Atf4tm1Tow
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Age:
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embryonic day 16.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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Not Specified
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: humerus |
Trace |
Regionally restricted |
5Ab'
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Authors report decreased expression in Atf4-/- growth plates. Expression barely detectable at E16.
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Specimen
5Ac:
postnatal day 0
(more )
Specimen
5Ac:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal day 0
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Sex:
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Not Specified
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Type:
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Not Specified
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: humerus |
Present |
Regionally restricted |
5Ac
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Expression in prehypertrophic chondrocytes increased at E16 and P0 in wildtype humeri.
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Specimen
5Ac':
(close )
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Genetic Background:
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involves: 129
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Mutant Allele(s):
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Atf4tm1Tow/Atf4tm1Tow
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Age:
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postnatal day 0
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Sex:
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Not Specified
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Type:
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Not Specified
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: humerus |
Weak |
Regionally restricted |
5Ac'
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Authors report decreased expression in Atf4-/- growth plates. Expression substantially lower than in wildtype.
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Analysis Tools