Antibody:	U2aCyt1
Assay notes:	Two antibodies were described in the publication; authors indicate they produce similar staining. The antibody used in this assay was not detailed; either MGI:6272749 or MGI:6272750 could have been used.

Structure	Level	Pattern	Image	Note
TS26: organ of Corti	Present	Regionally restricted	3A	Expression was detected all along the stereocilia of outer hair cells, whereas no labeling is found in the stereocilia of inner hair cells.

Structure	Level	Pattern	Image	Note
TS26: organ of Corti	Present	Regionally restricted		At E20, the outer hair cell labeling becomes restricted to the base of the stereocilia and also appears at the base of the inner hair cell sterocilia.

Structure	Level	Pattern	Image	Note
TS27: organ of Corti	Present	Regionally restricted		Intense labeling at the base of growing sterocilia persisted at P0.

Structure	Level	Pattern	Image	Note
TS28: organ of Corti	Present	Regionally restricted	3B left	Expression was detected at the base of the stereocilia of both outer and inner hair cells.

Structure	Level	Pattern	Image	Note
TS28: organ of Corti	Present	Regionally restricted	3B right	Expression was detected at the base of the stereocilia of inner hair cells.

Structure	Level	Pattern	Image	Note
TS28: organ of Corti	Present	Regionally restricted	3C	Labeling of sterocilia starts to fade out first in inner hair cells at P10.

Structure	Level	Pattern	Image	Note
TS28: organ of Corti	Strong	Regionally restricted	3D	Usherin is no longer detected in the sterocilia, but a strong labeling can be seen in the apical part of the cell soma in both hair cells and supporting cells.

Structure	Level	Pattern	Image	Note
TS28: inner ear	Present	Regionally restricted	3E P5	Expression was detected at the base of the stereocilia of vestibular hair cells.

Structure	Level	Pattern	Image	Note
TS28: inner ear	Present	Regionally restricted	3E P15	Expression was detected at the base of the stereocilia of vestibular hair cells.