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Gene Expression Data
Assay Details
Assay
Reference: J:262484 Inoue YU, et al., Generation of Pax6-IRES-EGFP knock-in mouse via the cloning-free CRISPR/Cas9 system to reliably visualize neurodevelopmental dynamics. Neurosci Res. 2018 Jan 31;
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6269407
Gene symbol: Pax6
Gene name: paired box 6
Antibody: anti-Pax6
Detection system: Secondary antibody coupled to Alexa Fluor
Results
Specimen 3B WT: postnatal day 2 (more )
Structure Level Pattern Image Note
TS27: retina ganglion cell layer Present 3B WT
TS27: retina inner nuclear layer Present 3B WT
Specimen 3B WT/IRES-EGFP: postnatal day 2; Pax6em1Yinn/Pax6+ (more )
Note: Double labeled: magenta - Pax6 protein; green - Pax6 reporter (assay MGI:6269401).
Structure Level Pattern Image Note
TS27: retina ganglion cell layer Present 3B +/- Pax6 , 3B +/- merge Pax6-positive cells mostly express EGFP at the appropriate intensities, while some of them express weaker/stronger EGFP than the endogenous levels.
TS27: retina inner nuclear layer Present 3B +/- Pax6 , 3B +/- merge Pax6-positive cells mostly express EGFP at the appropriate intensities, while some of them express weaker/stronger EGFP than the endogenous levels.
Specimen 3B IRES-EGFP/IRES-EGFP: postnatal day 2; Pax6em1Yinn/Pax6em1Yinn (more )
Note: Double labeled: magenta - Pax6 protein; green - Pax6 reporter (assay MGI:6269401).
Structure Level Pattern Image Note
TS27: retina ganglion cell layer Present 3B -/- Pax6 , 3B -/- merge EGFP expressions precisely recapitulated the endogenous expression profiles.
TS27: retina inner nuclear layer Present 3B -/- Pax6 , 3B -/- merge EGFP expressions precisely recapitulated the endogenous expression profiles.
Specimen 4B upper: embryonic day 12.5; Pax6em1Yinn/Pax6em1Yinn (more )
Structure Level Pattern Image Note
TS20: cerebral cortex Present Graded 4B upper left Mediolaterally graded, being most intense in the lateral side.
Specimen 4B lower: embryonic day 12.5; Pax6em1Yinn/Pax6em1Yinn (more )
Structure Level Pattern Image Note
TS20: cerebral cortex Present Graded 4B lower left Mediolaterally graded, being most intense in the lateral side.
Specimen S3A/B upper: postnatal day 2; Pax6em1Yinn/Pax6+ (more )
Note: Double labeled: magenta - Pax6 protein; green - Pax6 reporter (assay MGI:6269401).
Structure Level Pattern Image Note
TS27: retina ganglion cell layer Present S3A upper , S3B upper right , S3B upper left
TS27: retina inner nuclear layer Present S3A upper , S3B upper right , S3B upper left
Specimen S3A/B lower: postnatal day 2; Pax6em1Yinn/Pax6em1Yinn (more )
Note: Double labeled: magenta - Pax6 protein; green - Pax6 reporter (assay MGI:6269401).
Structure Level Pattern Image Note
TS27: retina ganglion cell layer Present S3B lower left , S3B lower right , S3A lower
TS27: retina inner nuclear layer Present S3B lower left , S3B lower right , S3A lower
Specimen S4A: postnatal day 2; Pax6em1Yinn/Pax6em1Yinn (more )
Note: Double labeled: magenta - Pax6 protein; green - Pax6 reporter (assay MGI:6269401).
Structure Level Pattern Image Note
TS27: cerebellum external granule cell layer Present S4A left , S4A right Expressed in most cells. Pax6 and EGFP expressed at the similar levels.
TS27: cerebellum molecular layer Present S4A left , S4A right Expressed in most cells. Pax6 and EGFP expressed at the similar levels.
Specimen S4B: postnatal day 80; Pax6em1Yinn/Pax6em1Yinn (more )
Note: Double labeled: magenta - Pax6 protein; green - Pax6 reporter (assay MGI:6269401).
Structure Level Pattern Image Note
TS28: testis Present S4B left , S4B right
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
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