Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:157035
Fisher CL, et al., Additional sex combs-like 1 belongs to the enhancer of trithorax and polycomb group and genetically interacts with Cbx2 in mice. Dev Biol. 2010 Jan 1;337(1):9-15
|
|
Assay type:
|
RNA in situ
|
|
MGI Accession ID:
|
MGI:6258759
|
|
Gene symbol:
|
Hoxa4
|
|
Gene name:
|
homeobox A4
|
|
Probe:
|
Hoxa4 probe
|
|
Probe preparation:
|
Antisense labelled with digoxigenin RNA
|
|
|
Results
|
Specimen
3 Hoxa4 +/+:
embryonic day 10.5
(more  )
Specimen
3 Hoxa4 +/+:
(close  )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 10.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS17: future spinal cord |
Present |
Regionally restricted |
3_Hoxa4 +/+
|
Anterior boundary of expression in ectoderm at the upper cervical spinal cord and spinal ganglia. Expression levels decrease caudally. Expression undetectable at thoracic level.
|
| TS17: spinal ganglion |
Present |
Regionally restricted |
3_Hoxa4 +/+
|
Anterior boundary of expression in ectoderm at the upper cervical spinal cord and spinal ganglia. Expression levels decrease caudally. Expression undetectable at thoracic level.
|
| TS17: paraxial mesenchyme |
Present |
Regionally restricted |
3_Hoxa4 +/+
|
Expression in mesoderm anteriorly to prevertebra 1. Expression is reduced posterior to prevertebra 6.
|
Specimen
3 Hoxa4 tm1Bc/tm1Bc:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd
|
|
Mutant Allele(s):
|
Asxl1tm1Bc/Asxl1tm1Bc
|
|
Age:
|
embryonic day 10.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS17: future spinal cord |
Present |
Regionally restricted |
3_Hoxa4 tm1Bc/tm1Bc
|
Authors state that in 5/7 homozygous mutant embryos, anterior boundaries of both ectodermal and mesodermal expression are shifted anteriorly by 1 or 2 somites relative to wild-type embryos. Two mutant embryos show expression in caudal mesoderm where it is never detected in the wild-type.
|
| TS17: paraxial mesenchyme |
Present |
Regionally restricted |
3_Hoxa4 tm1Bc/tm1Bc
|
Authors state that in 5/7 homozygous mutant embryos, anterior boundaries of both ectodermal and mesodermal expression are shifted anteriorly by 1 or 2 somites relative to wild-type embryos. Two mutant embryos show expression in caudal mesoderm where it is never detected in the wild-type.
|
|
Analysis Tools
