Gene Expression Data
Assay Details
Assay
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Reference:
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J:31081
Wilson DB, et al., Alterations in cranial morphogenesis in the Lp mutant mouse. J Craniofac Genet Dev Biol. 1995 Oct-Dec;15(4):182-9
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6258396
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Gene symbol:
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Ncam1
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Gene name:
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neural cell adhesion molecule 1
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Antibody:
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Anti-NCAM
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Detection system:
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Secondary antibody coupled to fluorescein
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Results
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Specimen
1F:
embryonic day 10.5
(more )
Note: This 'normal' specimen was either +/+ or Lp/+.
Specimen
1F:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Ste. Marie's
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Embedding:
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Paraffin
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Note:
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This 'normal' specimen was either +/+ or Lp/+.
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Specimen
1G:
embryonic day 10.5
(more )
Note: This 'normal' specimen was either +/+ or Lp/+.
Specimen
1G:
(close )
Genetic Background:
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Not Specified
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Age:
|
embryonic day 10.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Ste. Marie's
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Embedding:
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Paraffin
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Note:
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This 'normal' specimen was either +/+ or Lp/+.
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Structure |
Level |
Pattern |
Image |
Note |
TS17: notochord |
Present |
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1G
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TS17: head mesenchyme |
Ambiguous |
Regionally restricted |
1G
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The mesenchyme surrounding the neuroepithelium was poorly labeled.
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Specimen
2F:
(close )
Genetic Background:
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Not Specified
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Mutant Allele(s):
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Vangl2Lp/Vangl2Lp
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Age:
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embryonic day 10.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Ste. Marie's
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS17: hindbrain |
Present |
Regionally restricted |
2F
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In the mutant, expression was detected in the everted tip of the open neural fold.
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Specimen
2G:
(close )
Genetic Background:
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Not Specified
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Mutant Allele(s):
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Vangl2Lp/Vangl2Lp
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Age:
|
embryonic day 10.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Ste. Marie's
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS17: notochord |
Present |
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2G
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Expression is similar to normal in the mutant.
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TS17: head mesenchyme |
Ambiguous |
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2G
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The mesenchyme surrounding the neuroepithelium was poorly labeled. Expression is similar to normal in the mutant.
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Specimen
3C:
(close )
Genetic Background:
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Not Specified
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Mutant Allele(s):
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Vangl2Lp/Vangl2Lp
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Age:
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embryonic day 11.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Ste. Marie's
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS19: hindbrain |
Present |
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3C
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In the mutant, expression was similar to normal.
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Specimen
3D:
embryonic day 11.5
(more )
Note: This 'normal' specimen was either +/+ or Lp/+.
Specimen
3D:
(close )
Genetic Background:
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Not Specified
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Age:
|
embryonic day 11.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
|
Not Specified
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Type:
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section
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Fixation:
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Ste. Marie's
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Embedding:
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Paraffin
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Note:
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This 'normal' specimen was either +/+ or Lp/+.
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Specimen
4E:
(close )
Genetic Background:
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Not Specified
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Mutant Allele(s):
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Vangl2Lp/Vangl2Lp
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Age:
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embryonic day 12.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
Ste. Marie's
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS20: hindbrain |
Present |
Regionally restricted |
4E
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Expression was detected in the distal tips of the abnormal hindbrain.
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Specimen
4F:
embryonic day 12.5
(more )
Note: This 'normal' specimen was either +/+ or Lp/+.
Specimen
4F:
(close )
Genetic Background:
|
Not Specified
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Age:
|
embryonic day 12.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
|
Sex:
|
Not Specified
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Type:
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section
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Fixation:
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Ste. Marie's
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Embedding:
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Paraffin
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Note:
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This 'normal' specimen was either +/+ or Lp/+.
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Specimen
6A:
embryonic day 12.5
(more )
Note: This 'normal' specimen was either +/+ or Lp/+.
Specimen
6A:
(close )
Genetic Background:
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Not Specified
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Age:
|
embryonic day 12.5
|
Age Note:
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Age normalized so that noon of plug day = E0.5.
|
Sex:
|
Not Specified
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Type:
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section
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Fixation:
|
Ste. Marie's
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Embedding:
|
Paraffin
|
Note:
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This 'normal' specimen was either +/+ or Lp/+.
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: notochord |
Present |
Regionally restricted |
6A
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Expression was detected in the notochord, but the surrounding sheath was negative.
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Specimen
6B:
(close )
Genetic Background:
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Not Specified
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Mutant Allele(s):
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Vangl2Lp/Vangl2Lp
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Age:
|
embryonic day 12.5
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Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
Sex:
|
Not Specified
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Type:
|
section
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Fixation:
|
Ste. Marie's
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Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: notochord |
Present |
Regionally restricted |
6B
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As in the normal embryo, expression was detected in the notochord of the abnormal embryo, but the surrounding sheath was negative.
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