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Gene Expression Data
Assay Details
Assay
Reference: J:260421 Mesman S, et al., Expression analyzes of early factors in midbrain differentiation programs. Gene Expr Patterns. 2018 Jan;27:8-15
Assay type: RNA in situ
MGI Accession ID: MGI:6258331
Gene symbol: Hes5
Gene name: hes family bHLH transcription factor 5
Probe: Hes5 probe15
Probe preparation: labelled with digoxigenin
Visualized with: Alkaline phosphatase
Results
Specimen 5A E11.5 Lateral: embryonic day 11.5 (more )
Note: Double labeled: blue - Hes5; brown - Th (assay MGI:6258336).
Structure Level Pattern Image Note
TS19: midbrain Present Widespread 5A E11.5 Lateral Expression throughout entire midbrain.
TS19: hindbrain Present Regionally restricted 5A E11.5 Lateral Expression extends from rhombomere 1 to prosomere 3
TS19: prosomere 1 Present 5A E11.5 Lateral Expression extends from rhombomere 1 to prosomere 3
TS19: prosomere 2 Present 5A E11.5 Lateral Expression extends from rhombomere 1 to prosomere 3
TS19: prosomere 3 Present 5A E11.5 Lateral Expression extends from rhombomere 1 to prosomere 3
Specimen 5A E11.5 Medial: embryonic day 11.5 (more )
Note: Double labeled: blue - Hes5; brown - Th (assay MGI:6258336).
Structure Level Pattern Image Note
TS19: midbrain-hindbrain junction Absent 5A E11.5 Medial No expression in isthmic organizer.
Specimen 5A E12.5 Lateral: embryonic day 12.5 (more )
Note: Double labeled: blue - Hes5; brown - Th (assay MGI:6258336).
Structure Level Pattern Image Note
TS20: hindbrain Present Regionally restricted 5A E12.5 Lateral Expression extends from rhombomere 1 to prosomere 3
TS20: midbrain Present 5A E12.5 Lateral Expression extends from rhombomere 1 to prosomere 3
TS20: prosomere 1 Present 5A E12.5 Lateral Expression extends from rhombomere 1 to prosomere 3
TS20: prosomere 2 Present 5A E12.5 Lateral Expression extends from rhombomere 1 to prosomere 3
TS20: prosomere 3 Present 5A E12.5 Lateral Expression extends from rhombomere 1 to prosomere 3
Specimen 5A E12.5 Medial: embryonic day 12.5 (more )
Note: Double labeled: blue - Hes5; brown - Th (assay MGI:6258336).
Structure Level Pattern Image Note
TS20: midbrain-hindbrain junction Absent 5A E12.5 Medial No expression in isthmic organizer.
Specimen 5A1: embryonic day 12.5 (more )
Note: Double labeled: blue - Hes5; brown - Th (assay MGI:6258336).
Structure Level Pattern Image Note
TS20: midbrain Present Regionally restricted 5A1 Some overlap in expression with medially located Th-expressing neurons.
Specimen 5A E14.5 Lateral: embryonic day 14.5 (more )
Note: Double labeled: blue - Hes5; brown - Th (assay MGI:6258336).
Structure Level Pattern Image Note
TS22: midbrain Ambiguous 5A E14.5 Lateral
Specimen 5A E14.5 Medial: embryonic day 14.5 (more )
Note: Double labeled: blue - Hes5; brown - Th (assay MGI:6258336).
Structure Level Pattern Image Note
TS22: midbrain Present Regionally restricted 5A E14.5 Medial Some overlap in expression with medially located Th-expressing neurons.
Specimen 5A2: embryonic day 14.5 (more )
Note: Double labeled: blue - Hes5; brown - Th (assay MGI:6258336).
Structure Level Pattern Image Note
TS22: midbrain Present Regionally restricted 5A2 Some overlap in expression with medially located Th-expressing neurons.
Specimen 5B E11.5 Rostral: embryonic day 11.5 (more )
Note: Double labeled: blue - Hes5; brown - Th (assay MGI:6258336).
Structure Level Pattern Image Note
TS19: midbrain floor plate Present Regionally restricted 5B E11.5 Rostral Specific expression in ventricular zone of floor plate and basal plate of midbrain.
Specimen 5B E11.5 Caudal: embryonic day 11.5 (more )
Note: Double labeled: blue - Hes5; brown - Th (assay MGI:6258336).
Structure Level Pattern Image Note
TS19: midbrain ventricular layer Present Regionally restricted 5B E11.5 Caudal Specific expression in ventricular zone of floor plate and basal plate of midbrain.
Specimen 5B E12.5 Rostral: embryonic day 12.5 (more )
Note: Double labeled: blue - Hes5; brown - Th (assay MGI:6258336).
Structure Level Pattern Image Note
TS20: midbrain ventricular layer Present Regionally restricted 5B E12.5 Rostral Expression overlaps with few Th-expressing neurons in ventricular zone of midbrain, specific for floor plate region.
Specimen 5B E12.5 Caudal: embryonic day 12.5 (more )
Note: Double labeled: blue - Hes5; brown - Th (assay MGI:6258336).
Structure Level Pattern Image Note
TS20: midbrain ventricular layer Present Regionally restricted 5B E12.5 Caudal Specific expression in ventricular zone of floor plate and basal plate of midbrain.
Specimen 5B1: embryonic day 12.5 (more )
Note: Double labeled: blue - Hes5; brown - Th (assay MGI:6258336).
Structure Level Pattern Image Note
TS20: midbrain ventricular layer Present Regionally restricted 5B1 Expression overlaps with few Th-expressing neurons in ventricular zone of midbrain, specific for floor plate region.
Specimen 5B E14.5 Rostral: embryonic day 14.5 (more )
Note: Double labeled: blue - Hes5; brown - Th (assay MGI:6258336).
Structure Level Pattern Image Note
TS22: midbrain floor plate Weak Regionally restricted 5B E14.5 Rostral Expression overlaps with few Th-expressing neurons in ventricular zone of midbrain, specific for floor plate region.
Specimen 5B E14.5 Caudal: embryonic day 14.5 (more )
Note: Double labeled: blue - Hes5; brown - Th (assay MGI:6258336).
Structure Level Pattern Image Note
TS22: midbrain Ambiguous 5B E14.5 Caudal
Specimen 5B2: embryonic day 14.5 (more )
Note: Double labeled: blue - Hes5; brown - Th (assay MGI:6258336).
Structure Level Pattern Image Note
TS22: midbrain ventricular layer Weak Regionally restricted 5B2 Expression overlaps with few Th-expressing neurons in ventricular zone of midbrain, specific for floor plate region.
Specimen 5B Adult: postnatal adult (more )
Note: Double labeled: blue - Hes5; brown - Th (assay MGI:6258336).
Structure Level Pattern Image Note
TS28: midbrain Absent 5B Adult No expression in adult mdDA (mesodiencephalic dopaminergic) neuronal population.
Specimen 5B3: postnatal adult (more )
Note: Double labeled: blue - Hes5; brown - Th (assay MGI:6258336).
Structure Level Pattern Image Note
TS28: midbrain Absent 5B3 No expression in adult mdDA (mesodiencephalic dopaminergic) neuronal population.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
MGI 6.24 		The Jackson Laboratory