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Gene Expression Data
Assay Details
Assay
Reference: J:258304 Pui HP, et al., NANOS2 acts as an intrinsic regulator of gonocytes-to-spermatogonia transition in the murine testes. Mech Dev. 2018 Feb;149:27-40
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6257343
Gene symbol: Gfra1
Gene name: glial cell line derived neurotrophic factor family receptor alpha 1
Antibody: anti-GFRA1 (GT15004)
Detection system: Secondary antibody
Results
Specimen 4A I: embryonic day 17.5 (more )
Note: Double labeled: green - Gfra1; red - Stra8 (assay MGI:6257342).
Structure Level Pattern Image Note
TS25: germ cell of testis Present 4A I third , 4A I top , 4A I second Expression is in a subset of nascent spermatogonia.
Specimen 4A II: embryonic day 18.5 (more )
Note: Double labeled: green - Gfra1; red - Stra8 (assay MGI:6257342).
Structure Level Pattern Image Note
TS26: germ cell of testis Present 4A II top , 4A II third , 4A II second Expression is in a subset of nascent spermatogonia.
Specimen 4A III: postnatal day 0.5 (more )
Note: Double labeled: green - Gfra1; red - Stra8 (assay MGI:6257342).
Structure Level Pattern Image Note
TS27: spermatogonium Present 4A III second , 4A III top , 4A III third Expression is in a subset of nascent spermatogonia (84%).
Specimen 4A IV: postnatal day 1.5 (more )
Note: Double labeled: green - Gfra1; red - Stra8 (assay MGI:6257342).
Structure Level Pattern Image Note
TS27: spermatogonium Present 4A IV third , 4A IV top , 4A IV second Expression is in a subset of nascent spermatogonia.
Specimen 6F I Control: embryonic day 16.5 (more )
Note: Double labeled: black - Gfra1; green - Ddx4 (assay MGI:6257408).
Structure Level Pattern Image Note
TS24: germ cell of testis Trace 6F I Control Expression was rarely observed.
Specimen 6F N2KO: embryonic day 16.5; Nanos2tm1Ysa/Nanos2tm1Ysa (more )
Note: Double labeled: black - Gfra1; green - Ddx4 (assay MGI:6257408).
Structure Level Pattern Image Note
TS24: germ cell of testis Present 6F I N2KO In this mutant there was expression in about 15% of germ cells.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory