Antibody:	Anti-Filia
Detection system:	Secondary antibody coupled to Alexa Fluor 488

Structure	Level	Pattern	Image	Note
TS28: oocyte	Present		6_Merge Oocyte , 6_Filia Oocyte	Co-localization was noted in the subcortex.

Structure	Level	Pattern	Image	Note
TS28: secondary oocyte	Present		6_Merge Egg , 6_Filia Egg	Co-localization was noted in the subcortex.

Structure	Level	Pattern	Image	Note
TS1: 1-cell stage embryo	Present		6_Filia 1-Cell , 6_Merge 1-Cell

Structure	Level	Pattern	Image	Note
TS2: 2-cell stage embryo	Present	Regionally restricted	6_Filia 2-Cell , 6_Merge 2-Cell	Expression was peripherally localized.

Structure	Level	Pattern	Image	Note
TS3: 4-cell stage embryo	Present	Regionally restricted	6_Filia 4-Cell , 6_Merge 4-Cell	Expression was peripherally localized.

Structure	Level	Pattern	Image	Note
TS3: compacted morula	Present	Regionally restricted	6_Filia Morula , 6_Merge Morula	Expression was peripherally localized. The protein complex was not detected from the 'inner' cells.

Structure	Level	Pattern	Image	Note
TS4: inner cell mass	Present	Regionally restricted	6_Filia Blastocyst , 6_Merge Blastocyst	Expression was peripherally localized. The protein complex was not detected from the 'inner' cells.

Structure	Level	Pattern	Image	Note
TS3: compacted morula	Present	Regionally restricted	8C	Cell cleavage of blastomeres can be parallel or orthogonal to the axis of polarity. If parallel, the daughter cells are equivalent; if orthogonal, the 'outer' cells seemingly contain the Filia-Mater complex and 'inner' cells do not.