Slbp Western blot Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:149239 Zhang W, et al., Maternally encoded stem-loop-binding protein is degraded in 2-cell mouse embryos by the co-ordinated activity of two separately regulated pathways. Dev Biol. 2009 Apr 1;328(1):140-7
Assay type:	Western blot
MGI Accession ID:	MGI:6209466
Gene symbol:	Slbp
Gene name:	stem-loop binding protein

Antibody:	anti-SLBP
Detection system:	Secondary antibody coupled to horseradish peroxidase

Results

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	Size Not Specified	Genetic Background	Mutant Allele(s)	Sex
Met II	P adult	TS28: oocyte	Strong	CD-1		Not Specified
44 h	E1.33 (a)	TS2: 2-cell stage conceptus	Present (b)	CD-1		Not Specified
48 h	E1.5 (a)	TS2: 2-cell stage conceptus	Present (c)	CD-1		Not Specified
52 h	E1.67 (a)	TS2: 2-cell stage conceptus	Weak (d)	CD-1		Not Specified
54 h	E1.75 (a)	TS2: 2-cell stage conceptus	Weak	CD-1		Not Specified

Notes:
(a) Age based on time of collection, assuming fertilization occurred 12 hours post-hCG injection.
(b) Authors report expression had declined slightly, compared to amount present in unfertilized eggs, by 44 hours post-hCG injection.
(c) Expression decreased substantially over the next 8 hours.
(d) Authors state that 52 h embryos contain only about 1/4 the amount present in 44h embryos.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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