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Gene Expression Data
Assay Details
Assay
Reference: J:264681 Koehler S, et al., Construction of a viral T2A-peptide based knock-in mouse model for enhanced Cre recombinase activity and fluorescent labeling of podocytes. Kidney Int. 2017 Jun;91(6):1510-1517
Assay type: In situ reporter (knock in)
MGI Accession ID: MGI:6202340
Gene symbol: Nphs2
Gene name: nephrosis 2, podocin
Reporter: other (see notes)
Antibody: anti-2A
Assay notes: The T2A viral protein, included in the allele construct, was used as the reporter in this assay.
Results
Specimen 1e - 12 weeks: postnatal week 12; Nphs2tm1.1(icre)Ptb/Nphs2+ (more )
Note: Double labeled: purple - membrane-localized Nphs2 reporter (mTomato); green - nuclear-localized Nphs2 reporter (Cre; assay MGI:6200389).
Structure Level Pattern Image Note
TS28: Bowman's capsule of mature renal corpuscle Present Regionally restricted 1e 12 weeks mTomato , 1e 12 weeks merge Expression is seen in the podocytes.
Specimen 1e - 6 months: postnatal month 6; Nphs2tm1.1(icre)Ptb/Nphs2+ (more )
Note: Double labeled: purple - membrane-localized Nphs2 reporter (mTomato); green - nuclear-localized Nphs2 reporter (Cre; assay MGI:6200389).
Structure Level Pattern Image Note
TS28: Bowman's capsule of mature renal corpuscle Present Regionally restricted 1e 6 months merge , 1e 6 months mTomato Expression is seen in the podocytes.
Specimen 1e - 1 year: postnatal year 1; Nphs2tm1.1(icre)Ptb/Nphs2+ (more )
Note: Double labeled: purple - membrane-localized Nphs2 reporter (mTomato); green - nuclear-localized Nphs2 reporter (Cre; assay MGI:6200389).
Structure Level Pattern Image Note
TS28: Bowman's capsule of mature renal corpuscle Present Regionally restricted 1e 1 year mTomato , 1e 1 year merge Expression is seen in the podocytes.
Specimen 3c - 12 weeks: postnatal week 12; Nphs2tm1.1(icre)Ptb/Nphs2+ (more )
Note: Double labeled: purple - Nphs2 reporter; green - Nphs2 protein (assay MGI:6202121).
Structure Level Pattern Image Note
TS28: Bowman's capsule of mature renal corpuscle Present Regionally restricted 3c left , 3d left Expression is seen in the podocytes.
Specimen 3c - 6 months: postnatal month 6; Nphs2tm1.1(icre)Ptb/Nphs2+ (more )
Note: Double labeled: purple - Nphs2 reporter; green - Nphs2 protein (assay MGI:6202121).
Structure Level Pattern Image Note
TS28: Bowman's capsule of mature renal corpuscle Present Regionally restricted 3d center , 3c center Expression is seen in the podocytes.
Specimen 3c - 1 year: postnatal year 1; Nphs2tm1.1(icre)Ptb/Nphs2+ (more )
Note: Double labeled: purple - Nphs2 reporter; green - Nphs2 protein (assay MGI:6202121).
Structure Level Pattern Image Note
TS28: Bowman's capsule of mature renal corpuscle Present Regionally restricted 3c right , 3d right Expression is seen in the podocytes.
Specimen 4d - upper: postnatal day 19; Phb2tm1Tlan/Phb2+, Nphs2tm1.1(icre)Ptb/Nphs2+ (more )
Note: Conditional mutant. Double labeled: purple - Nphs2 reporter; green - Nphs2 protein (assay MGI:6202121).
Structure Level Pattern Image Note
TS28: Bowman's capsule of mature renal corpuscle Present 4d upper right , 4d upper center
Specimen 4d - lower: postnatal day 19; Phb2tm1Tlan/Phb2tm1Tlan, Nphs2tm1.1(icre)Ptb/Nphs2+ (more )
Note: Conditional mutant. Double labeled: purple - Nphs2 reporter; green - Nphs2 protein (assay MGI:6202121).
Structure Level Pattern Image Note
TS28: Bowman's capsule of mature renal corpuscle Present 4d lower right , 4d lower center Expression is comparable in the mutant and the control.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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