Antibody:	Ab79
Detection system:	Secondary antibody coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS14: neural tube floor plate	Present		5D	Expression was accumulated at the luminal surface of floor plate cells.
TS14: notochord	Present		5D	Expression was detected on the surface of notochord cells.

Structure	Level	Pattern	Image	Note
TS14: neural tube floor plate	Present		5E	Double labeling indicates that peptides are only detected on, or very close to, the surface of cells transcribing Shh.
TS14: notochord	Present		5E	Double labeling indicates that peptides are only detected on, or very close to, the surface of cells transcribing Shh.

Structure	Level	Pattern	Image	Note
TS15: neural tube floor plate	Present		5F	Expression was detected on the surface of expressing cells and accumulated mostly at the ventricular and, to a lesser extent, basal surface.
TS15: future spinal cord	Present	Regionally restricted	5F	Expression appears to extend ventrolateral to the morphologically defined floor plate.

Structure	Level	Pattern	Image	Note
TS15: neural tube floor plate	Present		5G	Expression was detected on the surface of expressing cells and accumulated mostly at the ventricular and, to a lesser extent, basal surface.
TS15: future spinal cord	Present	Regionally restricted	5G	Expression appears to extend ventrolateral to the morphologically defined floor plate.

Structure	Level	Pattern	Image	Note
TS17: future spinal cord	Present	Regionally restricted	5I	Immunostaining became widely dispersed beyond the floor plate cells transcribing Shh.