Probe:	Plp/DM20 probe
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS15: diencephalon lateral wall	Present	Regionally restricted	1A	Expressed in the lateral basal plate, occupying entire thickness of the germinal layer. Neither the ventral paramedian zone nor the alar plate were labeled.
TS15: trigeminal preganglion	Present		1A

Structure	Level	Pattern	Image	Note
TS20: diencephalon lateral wall mantle layer	Present		1B
TS20: trigeminal ganglion	Present		1B
TS20: diencephalon lateral wall ventricular layer	Absent		1B

Structure	Level	Pattern	Image	Note
TS20: zona limitans intrathalamica	Present	Regionally restricted	2A	A linear transverse domain at the P2/P3 interprosomeric boundary.
TS20: telencephalon	Present	Regionally restricted	2A	Expressed in the anterior entopeduncular area, adjacent to the medial ganglionic eminence in the basal telencephalon.
TS20: cerebellum primordium	Present		2A
TS20: trigeminal ganglion	Strong		2A	Also expressed in its three roots.

Structure	Level	Pattern	Image	Note
TS20: zona limitans intrathalamica	Present	Regionally restricted	2B	A linear transverse domain at the P2/P3 interprosomeric boundary.
TS20: telencephalon	Present	Regionally restricted	2B	Expressed in the anterior entopeduncular area, adjacent to the medial ganglionic eminence in the basal telencephalon.
TS20: facial nerve	Present		2B
TS20: olfactory migratory mass	Present		2B
TS20: vestibulocochlear nerve	Present		2B
TS20: diencephalon lateral wall	Strong	Regionally restricted	2B	Expressed in a second axis extended rostrally from the lateral basal plate of the diencephalon, along the basal plate of the hypothalamus (mammillary and tuberal regions).
TS20: hypothalamus	Strong	Regionally restricted	2B	Expressed in a second axis extended rostrally from the lateral basal plate of the diencephalon, along the basal plate of the hypothalamus (mammillary and tuberal regions).
TS20: mammillary area	Strong		2B
TS20: trigeminal ganglion	Strong		2B	Also expressed in its three roots.

Structure	Level	Pattern	Image	Note
TS20: zona limitans intrathalamica	Present	Regionally restricted	2C	A linear transverse domain at the P2/P3 interprosomeric boundary.
TS20: olfactory migratory mass	Present		2C
TS20: spinal nerve	Present		2C
TS20: hypothalamus	Present	Regionally restricted	2C	Expressed in the ventral paramedian zone.

Structure	Level	Pattern	Image	Note
TS20: telencephalon	Present	Regionally restricted	2D	Expressed in the anterior entopeduncular area, adjacent to the medial ganglionic eminence in the basal telencephalon.
TS20: diencephalon lateral wall	Strong	Regionally restricted	2D	Expressed in a second axis extended rostrally from the lateral basal plate of the diencephalon, along the basal plate of the hypothalamus (mammillary and tuberal regions).
TS20: hypothalamus	Strong	Regionally restricted	2D	Expressed in the lateral basal plate.
TS20: mammillary area	Strong		2D

Structure	Level	Pattern	Image	Note
TS20: telencephalon	Present	Regionally restricted	2E	Expressed in the anterior entopeduncular area, adjacent to the medial ganglionic eminence in the basal telencephalon.
TS20: spinal cord	Present	Regionally restricted	2E	Expressed in the sensory and motor roots.
TS20: spinal ganglion	Present	Regionally restricted	2E	In some cells.
TS20: sympathetic ganglion	Present	Regionally restricted	2E	Expressed in the prevertebral and perivascular ganglia.

Structure	Level	Pattern	Image	Note
TS20: spinal cord	Present	Regionally restricted	2F	Expressed in the sensory and motor roots.
TS20: spinal nerve	Present		2F
TS20: spinal ganglion	Present	Regionally restricted	2F	In some cells.
TS20: sympathetic ganglion	Present	Regionally restricted	2F	Expressed in the prevertebral and perivascular ganglia.
TS20: peripheral nerve	Present	Regionally restricted	2F	Expressed along the pathways of the peripheral nerves in the limb buds and the tail.

Structure	Level	Pattern	Image	Note
TS22: diencephalon lateral wall	Strong	Regionally restricted	3A	Expressed in a second axis extended rostrally from the lateral basal plate of the diencephalon, along the basal plate of the hypothalamus (mammillary and tuberal regions).
TS22: hypothalamus lateral zone	Strong	Regionally restricted	3A	Expressed in a second axis extended rostrally from the lateral basal plate of the diencephalon, along the basal plate of the hypothalamus (mammillary and tuberal regions).
TS22: mammillary area	Strong		3A
TS22: amygdala	Present	Regionally restricted	3A	Expressed in a restricted zone in the medial amygdala.
TS22: hypothalamus	Present	Regionally restricted	3A	Expressed in the ventral hypothalamus.
TS22: trigeminal ganglion	Present		3A

Structure	Level	Pattern	Image	Note
TS22: diencephalon lateral wall ventricular layer	Trace		3B	Expression had largely disappeared.
TS22: diencephalon lateral wall mantle layer	Present		3B
TS22: diencephalon subventricular zone	Present		3B
TS22: diencephalon lateral wall	Present	Regionally restricted	3B	Expressed at the posterior part of the basal diencephalon outside a halo of densely packed cells surrounding the third ventricle.

Structure	Level	Pattern	Image	Note
TS27: diencephalon	Strong	Regionally restricted	3C	Expressed in a second axis extended rostrally from the lateral basal plate of the diencephalon, along the basal plate of the hypothalamus (mammillary and tuberal regions).
TS27: hypothalamus lateral zone	Strong	Regionally restricted	3C	Expressed in a second axis extended rostrally from the lateral basal plate of the diencephalon, along the basal plate of the hypothalamus (mammillary and tuberal regions).
TS27: hypothalamus medial zone tuberal area	Strong		3C
TS27: mammillary area	Strong		3C
TS27: hypothalamus	Present	Regionally restricted	3C	Expressed in the ventral hypothalamus.
TS27: amygdala	Present		3C

Structure	Level	Pattern	Image	Note
TS27: diencephalon	Present	Regionally restricted	3D	Expressed in the eminentia media at the level of the third ventricle and in tracts such as the medial forebrain bundle.

Structure	Level	Pattern	Image	Note
TS27: cerebral internal capsule	Present		3E	Expressed in the telencephalic internal capsule.
TS27: cerebral hemisphere white matter	Present	Regionally restricted	3E	Expressed in subcortical white matter.
TS27: telencephalon	Present	Regionally restricted	3E	Expressed in the supralenticular internal capsule and the subcortical white matter, extending dorsomedially toward the corpus callosum and ventrolaterally along the capsula extrema, deep into the piriform cortex.

Structure	Level	Pattern	Image	Note
TS20: diencephalon lateral wall	Present	Regionally restricted	4A	Expressed in a more internal and dorsal position than the Tubb3+ neuronal cell bodies. The most ventral and external portion of the Plp1+ group of cells overlapped with the most dorsally and internally localized neurons.

Structure	Level	Pattern	Image	Note
TS22: pons	Present		4D	Did not colocalize with Tubb3-positive neuronal cell bodies.

Structure	Level	Pattern	Image	Note
TS22: diencephalon lateral wall mantle layer	Present		6A
TS22: hindbrain	Present	Regionally restricted	6A	Expressed in the caudal rhombencephalon.
TS22: pons	Present	Regionally restricted	6A	Formed two sparse columns on either side of the central canal.

Structure	Level	Pattern	Image	Note
TS25: spinal cord gray matter	Present	Scattered	6B
TS25: spinal cord white matter	Present	Scattered	6B
TS25: brainstem	Present	Scattered	6B	Scattered throughout the white and gray matter.
TS25: pons	Present	Scattered	6B	Scattered throughout the white and gray matter.

Structure	Level	Pattern	Image	Note
TS22: medulla oblongata	Present	Regionally restricted	6C	Formed two sparse columns on either side of the central canal at or near the basal plate. Cells were more numerous, and some were located at a short distance from the ventricular zone.

Structure	Level	Pattern	Image	Note
TS27: pons	Present		6D
TS27: ventral tegmental area	Present		6D
TS27: cerebellum white matter	Present	Regionally restricted	6D	Expressed in the deep white matter tract.
TS27: trigeminal nerve	Present	Regionally restricted	6D	Expressed in the descending trigeminal nerves.

Structure	Level	Pattern	Image	Note
TS22: spinal cord	Present	Regionally restricted	7A	Expressed in the ependymal layer in contact with the lumen, restricted to the ventral basal region. Formed two sparse columns of a single layer of cells on either side of the central canal.
TS22: spinal cord floor plate	Absent		7A

Structure	Level	Pattern	Image	Note
TS22: spinal cord	Present	Regionally restricted	7B	Expressed in the ependymal layer in contact with the lumen, restricted to the ventral basal region. Formed two sparse columns on either side of the central canal.
TS22: spinal cord floor plate	Absent		7B

Structure	Level	Pattern	Image	Note
TS27: spinal cord marginal layer	Strong	Regionally restricted	7D	Expressed in the ventral marginal layer.
TS27: nerve fiber tract	Strong	Regionally restricted	7D	Expressed in the superficial ventral tracts.
TS27: spinal cord	Strong	Regionally restricted	7D	Expressed in the ventral marginal layer. No expression in the superficial laminae of the dorsal horn.

Structure	Level	Pattern	Image	Note
TS22: olfactory epithelium	Present	Regionally restricted	8A	Expressed in cells associated with the superficial axonal layers of the olfactory bulb.
TS22: main olfactory bulb olfactory nerve layer	Present		8A
TS22: vomeronasal organ	Absent		8A

Structure	Level	Pattern	Image	Note
TS27: main olfactory bulb olfactory nerve layer	Present		8B
TS27: vomeronasal organ	Absent		8B

Structure	Level	Pattern	Image	Note
TS13: embryo	Absent

Structure	Level	Pattern	Image	Note
TS20: hindbrain	Present	Regionally restricted		Expressed in the caudal rhombencephalon.

Structure	Level	Pattern	Image	Note
TS22: main olfactory bulb ventricular layer	Absent			No expression in the secondary olfactory neurons.
TS22: spinal cord	Present			Did not colocalize with Tubb3- or Adgre1-positive cells.

Structure	Level	Pattern	Image	Note
TS25: diencephalon mantle layer	Present	Regionally restricted		Increased expression along the external layer of the mantle zone. Some positive cells were already seen inside, or in the close vicinity, of the major fiber bundles.
TS25: main olfactory bulb olfactory nerve layer	Present
TS25: vomeronasal organ	Absent

Structure	Level	Pattern	Image	Note
TS27: telencephalon subventricular zone	Present