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Gene Expression Data
Assay Details
Assay
Reference: J:149227 Liu Y, et al., Abnormal development of the neuromuscular junction in Nedd4-deficient mice. Dev Biol. 2009 Jun 1;330(1):153-66
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6200608
Gene symbol: Syt2
Gene name: synaptotagmin II
Antibody: anti-synaptotagmin-2
Detection system: Secondary antibody coupled to fluorescein
Results
Specimen 3A: embryonic day 13.5 (more )
Structure Level Pattern Image Note
TS21: phrenic nerve Present Regionally restricted 3A Authors state the secondary (intramuscular) branch of the primary phrenic nerve develops as a single bundle perpendicular to the long axis of the muscle fibers
Specimen 3B: embryonic day 13.5; Nedd4Gt(OST16211)Lex/Nedd4Gt(OST16211)Lex (more )
Structure Level Pattern Image Note
TS21: phrenic nerve Present Regionally restricted 3B Authors report secondary nerve branches are markedly defasciculated, often developing abnormal loops.
Specimen 3C: embryonic day 15.5 (more )
Structure Level Pattern Image Note
TS23: phrenic nerve Present Regionally restricted 3C Authors state stereotypic pattern of expression established at E13.5 is maintained throughout embryonic development. Ventral portion of diaphragm is fully innervated.
Specimen 3D: embryonic day 15.5; Nedd4Gt(OST16211)Lex/Nedd4Gt(OST16211)Lex (more )
Structure Level Pattern Image Note
TS23: phrenic nerve Present Regionally restricted 3D Authors report secondary nerve branches are markedly defasciculated, often developing abnormal loops. Nerves fail to innervate entire diaphragm, leaving an uninnervated gap in ventreal protion of diaphragm.
Specimen 3E: embryonic day 18.5 (more )
Structure Level Pattern Image Note
TS26: phrenic nerve Present Regionally restricted 3E Authors state stereotypic pattern of expression established at E13.5 is maintained throughout embryonic development. Numerous tertiary nerves extend from secondary branches and terminate within central region of muscle.
TS26: diaphragm Present Regionally restricted 3E Expression in ingrowing sensory nerves at edge of diaphragm.
Specimen 3F: embryonic day 18.5; Nedd4Gt(OST16211)Lex/Nedd4Gt(OST16211)Lex (more )
Structure Level Pattern Image Note
TS26: phrenic nerve Present Regionally restricted 3F Authors report secondary nerve branches are markedly defasciculated, often developing abnormal loops.
Specimen 3G: embryonic day 18.5 (more )
Structure Level Pattern Image Note
TS26: phrenic nerve Present Regionally restricted 3G Ventral portion of diaphragm is fully innervated.
Specimen 3H: embryonic day 18.5; Nedd4Gt(OST16211)Lex/Nedd4Gt(OST16211)Lex (more )
Structure Level Pattern Image Note
TS26: phrenic nerve Present Regionally restricted 3H Authors report secondary nerve branches are markedly defasciculated. Nerves fail to innervate entire diaphragm, leaving an uninnervated gap in ventreal protion of diaphragm.
Specimen 5D +/+: embryonic day 18.5 (more )
Note: Double labeled: green - Syt2 and Nefm; red - alpha-bungarotoxin.
Structure Level Pattern Image Note
TS26: diaphragm Present Regionally restricted 5D +/+ nerve , 5D +/+ merge Expression in nerve terminals.
Specimen 5D -/-: embryonic day 18.5; Nedd4Gt(OST16211)Lex/Nedd4Gt(OST16211)Lex (more )
Note: Double labeled: green - Syt2 and Nefm; red - alpha-bungarotoxin.
Structure Level Pattern Image Note
TS26: diaphragm Present Regionally restricted 5D -/- nerve , 5D -/- merge Expression in nerve terminals.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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12/30/2025
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