Vwa8 RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:264395 Grewe BS, et al., The spatial and developmental expression of mouse Vwa8 (von Willebrand domain-containing protein 8). Gene Expr Patterns. 2018 Apr 13;29:39-46
Assay type:	RT-PCR
MGI Accession ID:	MGI:6199696
Gene symbol:	Vwa8
Gene name:	von Willebrand factor A domain containing 8

Probe:	Vwa8 3030F, Vwa8 3428R
Probe preparation:	labelled with SYBR green
Assay notes:	Quantitative RT-PCR. Primers were specific to isoform Vwa8a. Samples were normalized relative to P7 spleen expression.

Results

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	398 bp	Amount	Genetic Background	Mutant Allele(s)	Sex
e11	E11.0 (a)	TS18: embryo	Present (b)	1 µg; total RNA	C57BL/6J		Not Specified
e12	E12.0 (a)	TS20: embryo	Present	1 µg; total RNA	C57BL/6J		Not Specified
e13	E13.0 (a)	TS21: embryo	Present	1 µg; total RNA	C57BL/6J		Not Specified
e16	E16.0 (a)	TS24: embryo	Present (c)	1 µg; total RNA	C57BL/6J		Not Specified
e18	E18.0 (a)	TS26: embryo	Present (d)	1 µg; total RNA	C57BL/6J		Not Specified
P0.5	P0.5	TS27: mouse	Present (e)	1 µg; total RNA	C57BL/6J		Not Specified

Notes:
(a) Age of embryo at noon of plug day not specified in reference.
(b) The Vwa8a expression at E11.0 was approximately 6-fold greater relative to P7 spleen.
(c) Expression decreased to below P7 spleen expression level at E16.0.
(d) Expression at E18.0 was above P7 spleen expression level.
(e) P0.5 expression level was below P7 spleen level.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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