Gene Expression Data
Assay Details
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Assay
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Reference:
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J:26506
Vielkind U, et al., Dynamics of Merkel cell patterns in developing hair follicles in the dorsal skin of mice, demonstrated by a monoclonal antibody to mouse keratin 8. Acta Anat (Basel). 1995;152(2):93-109
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6199447
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Gene symbol:
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Krt8
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Gene name:
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keratin 8
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Antibody:
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TROMA 1
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Detection system:
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Secondary antibody coupled to fluorescein
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Assay notes:
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Dorsolateral skin was examined in all cases. Tabby mutants could have been Ta/ta or Ta/Y.
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Results
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Specimen
1C:
embryonic day 13.0
(more  )
Note: Prefollicle stage.
Specimen
1C:
(close  )
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Genetic Background:
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C57BL/6J-Aw-J/JGbms
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Age:
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embryonic day 13.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Prefollicle stage.
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Specimen
2B:
embryonic day 14.0
(more )
Note: Tylotrich follicle stage 1 (placode).
Specimen
2B:
(close )
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Genetic Background:
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C57BL/6J-Aw-J/JGbms
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Age:
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embryonic day 14.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Tylotrich follicle stage 1 (placode).
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Specimen
3B:
embryonic day 15.0
(more )
Note: Tylotrich follicle stage 2 (hair plug).
Specimen
3B:
(close )
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Genetic Background:
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C57BL/6J-Aw-J/JGbms
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Age:
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embryonic day 15.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Tylotrich follicle stage 2 (hair plug).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: periderm |
Strong |
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3B
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| TS23: hair follicle |
Present |
Single cells |
3B
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Expression is in Merkel cells.
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| TS23: epidermis |
Present |
Single cells |
3B
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Expression is in a few solitary cells in the basal layer immediately adjacent to the hair plugs.
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Specimen
3C:
embryonic day 15.0
(more )
Note: Tylotrich follicle stage 2 (hair plug).
Specimen
3C:
(close )
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Genetic Background:
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C57BL/6J-Aw-J/JGbms
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Age:
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embryonic day 15.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Tylotrich follicle stage 2 (hair plug).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: periderm |
Present |
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3C
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| TS23: hair follicle |
Present |
Single cells |
3C
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Expression is in Merkel cells.
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| TS23: epidermis |
Present |
Single cells |
3C
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Expression is in a few solitary cells in the basal layer immediately adjacent to the hair plugs.
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Specimen
4B:
embryonic day 16.0
(more )
Note: Tylotrich follicle stage 3a (early dermal papillae).
Specimen
4B:
(close )
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Genetic Background:
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C57BL/6J-Aw-J/JGbms
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Age:
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embryonic day 16.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Tylotrich follicle stage 3a (early dermal papillae).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: periderm |
Strong |
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4B
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| TS24: hair follicle |
Present |
Regionally restricted |
4B
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Expression is in Merkel cells.
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| TS24: epidermis |
Present |
Clusters |
4B
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Expression is in Merkel cells within the basal epidermal layer caudal to the tylotrich follicle forming a disc-shaped structure.
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Specimen
4C:
embryonic day 16.0
(more )
Note: Tylotrich follicle stage 3a (early dermal papillae).
Specimen
4C:
(close )
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Genetic Background:
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C57BL/6J-Aw-J/JGbms
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Age:
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embryonic day 16.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Tylotrich follicle stage 3a (early dermal papillae).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: periderm |
Strong |
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4C
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| TS24: hair follicle |
Present |
Regionally restricted |
4C
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Expression is in Merkel cells.
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| TS24: epidermis |
Present |
Clusters |
4C
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Expression is in Merkel cells within the basal epidermal layer caudal to the tylotrich follicle forming a disc-shaped structure.
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Specimen
4D:
embryonic day 16.0
(more )
Note: Tylotrich follicle stage 3a (early dermal papillae).
Specimen
4D:
(close )
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Genetic Background:
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C57BL/6J-Aw-J/JGbms
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Age:
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embryonic day 16.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Tylotrich follicle stage 3a (early dermal papillae).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: periderm |
Strong |
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4D
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| TS24: hair follicle |
Present |
Regionally restricted |
4D
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Expression is in Merkel cells.
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| TS24: epidermis |
Present |
Regionally restricted |
4D
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Expression is in Merkel cells within the basal epidermal layer caudal to the tylotrich follicle forming a disc-shaped structure.
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Specimen
5B:
embryonic day 17.0
(more )
Note: Tylotrich follicle stage 3b (developing dermal papillae).
Specimen
5B:
(close )
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Genetic Background:
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C57BL/6J-Aw-J/JGbms
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Age:
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embryonic day 17.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Tylotrich follicle stage 3b (developing dermal papillae).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: periderm |
Present |
Regionally restricted |
5B
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| TS25: hair follicle |
Present |
Single cells |
5B
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Expression is in Merkel cells deep in the follicle.
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| TS25: epidermis |
Present |
Clusters |
5B
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Expression is in Merkel cells within the basal epidermal layer caudal to the tylotrich follicle forming a disc-shaped structure.
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Specimen
6B:
embryonic day 18.0
(more )
Note: Tylotrich follicle stage 3c (completed dermal papillae).
Specimen
6B:
(close )
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Genetic Background:
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C57BL/6J-Aw-J/JGbms
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Age:
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embryonic day 18.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Tylotrich follicle stage 3c (completed dermal papillae).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: hair follicle |
Present |
Single cells |
6B
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Expression is in Merkel cells deep in the follicle.
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| TS26: epidermis |
Present |
Clusters |
6B
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Expression is in Merkel cells within the basal epidermal layer caudal to the tylotrich follicle forming a disc-shaped structure.
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Specimen
7B:
embryonic day 19.0
(more )
Note: Tylotrich follicle stage 4 (early inner root sheath cone).
Specimen
7B:
(close )
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Genetic Background:
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C57BL/6J-Aw-J/JGbms
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Age:
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embryonic day 19.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Tylotrich follicle stage 4 (early inner root sheath cone).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: epidermis |
Present |
Regionally restricted |
7B
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Expression is caudal to the tylotrich follicle forming a disc-shaped structure.
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| TS26: hair follicle |
Present |
Regionally restricted |
7B
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Expression is caudal to the tylotrich follicle forming a disc-shaped structure.
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| TS26: hair outer root sheath |
Present |
Single cells |
7B
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Expression is in a few isolated Merkel cells.
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Specimen
7D:
embryonic day 19.0
(more )
Note: Tylotrich follicle stage 4 (early inner root sheath cone).
Specimen
7D:
(close )
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Genetic Background:
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C57BL/6J-Aw-J/JGbms
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Age:
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embryonic day 19.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Tylotrich follicle stage 4 (early inner root sheath cone).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: epidermis |
Present |
Regionally restricted |
7D
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Expression is caudal to the tylotrich follicle forming a disc-shaped structure.
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| TS26: hair follicle |
Present |
Regionally restricted |
7D
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Expression is caudal to the tylotrich follicle forming a disc-shaped structure.
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| TS26: hair outer root sheath |
Present |
Single cells |
7D
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Expression is in a few isolated Merkel cells.
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Specimen
7F:
embryonic day 19.0
(more )
Note: Tylotrich follicle stage 4 (early inner root sheath cone).
Specimen
7F:
(close )
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Genetic Background:
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C57BL/6J-Aw-J/JGbms
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Age:
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embryonic day 19.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Tylotrich follicle stage 4 (early inner root sheath cone).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: epidermis |
Present |
Regionally restricted |
7F
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Expression is caudal to the tylotrich follicle forming a disc-shaped structure.
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Specimen
not shown E14:
embryonic day 14.0; EdaTa/ EdaTa
(more )
Specimen
not shown E14:
(close )
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Genetic Background:
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B6.Cg-Aw-J EdaTa/JGbms
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Mutant Allele(s):
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EdaTa/EdaTa
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Age:
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embryonic day 14.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: periderm |
Present |
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| TS22: epidermis |
Present |
Regionally restricted |
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Expression is in the periderm and is absent in the rest of the epidermis.
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Specimen
10B:
embryonic day 16.0; EdaTa/ EdaTa
(more )
Specimen
10B:
(close )
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Genetic Background:
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B6.Cg-Aw-J EdaTa/JGbms
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Mutant Allele(s):
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EdaTa/EdaTa
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Age:
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embryonic day 16.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: periderm |
Present |
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10B
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| TS24: epidermis |
Present |
Regionally restricted |
10B
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Expression is in the periderm and is absent in the rest of the epidermis.
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Specimen
11B:
embryonic day 17.0; EdaTa/ EdaTa
(more )
Note: Awl follicle stage 2.
Specimen
11B:
(close )
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Genetic Background:
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B6.Cg-Aw-J EdaTa/JGbms
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Mutant Allele(s):
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EdaTa/EdaTa
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Age:
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embryonic day 17.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Awl follicle stage 2.
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Specimen
12B:
embryonic day 18.0; EdaTa/ EdaTa
(more )
Note: Awl follicle stage 3a.
Specimen
12B:
(close )
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Genetic Background:
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B6.Cg-Aw-J EdaTa/JGbms
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Mutant Allele(s):
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EdaTa/EdaTa
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Age:
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embryonic day 18.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Awl follicle stage 3a.
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Specimen
not shown E19:
embryonic day 19.0; EdaTa/ EdaTa
(more )
Note: Awl follicle stage 3b.
Specimen
not shown E19:
(close )
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Genetic Background:
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B6.Cg-Aw-J EdaTa/JGbms
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Mutant Allele(s):
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EdaTa/EdaTa
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Age:
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embryonic day 19.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Awl follicle stage 3b.
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Specimen
13B:
embryonic day 15.0; dny/ dny
(more )
Note: Tylotrich follicle stage 2.
Specimen
13B:
(close )
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Genetic Background:
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C57BL/6J-Aw-J dny/Gbms
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Mutant Allele(s):
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dny/dny
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Age:
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embryonic day 15.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Tylotrich follicle stage 2.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: hair follicle |
Present |
Single cells |
13B
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Expression is in Merkel cells in the upper part of the hair plug. Expression in this mutant is the same as in wild-type.
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Specimen
14B:
embryonic day 17.0; dny/ dny
(more )
Note: Tylotrich follicle stage 3b.
Specimen
14B:
(close )
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Genetic Background:
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C57BL/6J-Aw-J dny/Gbms
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Mutant Allele(s):
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dny/dny
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Age:
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embryonic day 17.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Tylotrich follicle stage 3b.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: epidermis |
Present |
Clusters |
14B
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Expression is in a cluster of cells in and just above the basal epidermal layer associated with the follicle. Expression in this mutant is the same as in wild-type.
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