Gene Expression Data
Assay Details
Assay
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Reference:
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J:145977
Tetreault N, et al., The LIM homeobox transcription factor Lhx2 is required to specify the retina field and synergistically cooperates with Pax6 for Six6 trans-activation. Dev Biol. 2009 Mar 15;327(2):541-50
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:6198683
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Gene symbol:
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Lhx2
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Gene name:
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LIM homeobox protein 2
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Probe:
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Lhx2 probe11
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
1A left:
embryonic day 8.0
(more )
Note: 2-somite stage
Specimen
1A left:
(close )
Genetic Background:
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either: 129 or CD-1
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Age:
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embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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2-somite stage
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Structure |
Level |
Pattern |
Image |
Note |
TS12: eye neural ectoderm |
Present |
Regionally restricted |
1A left
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At 2 somite stage, expression in anterior neural plate in region corresponding to prospective retina field.
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Specimen
1A center:
embryonic day 8.0
(more )
Note: 2-somite stage
Specimen
1A center:
(close )
Genetic Background:
|
either: 129 or CD-1
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Age:
|
embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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2-somite stage
|
Structure |
Level |
Pattern |
Image |
Note |
TS12: eye neural ectoderm |
Present |
Regionally restricted |
1A center
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At 2 somite stage, expression in anterior neural plate in region corresponding to prospective retina field.
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Specimen
1A right:
embryonic day 8.0
(more )
Note: 2-somite stage
Specimen
1A right:
(close )
Genetic Background:
|
either: 129 or CD-1
|
Age:
|
embryonic day 8.0
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
|
4% Paraformaldehyde
|
Note:
|
2-somite stage
|
Structure |
Level |
Pattern |
Image |
Note |
TS12: eye neural ectoderm |
Present |
Regionally restricted |
1A right
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At 2 somite stage, expression in anterior neural plate in region corresponding to prospective retina field.
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Specimen
1B left:
embryonic day 9.0
(more )
Specimen
1B left:
(close )
Genetic Background:
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either: 129 or CD-1
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Age:
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embryonic day 9.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
1B center:
embryonic day 9.0
(more )
Specimen
1B center:
(close )
Genetic Background:
|
either: 129 or CD-1
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Age:
|
embryonic day 9.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
1B right:
embryonic day 9.0
(more )
Specimen
1B right:
(close )
Genetic Background:
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either: 129 or CD-1
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Age:
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embryonic day 9.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
1C:
embryonic day 12.5
(more )
Specimen
1C:
(close )
Genetic Background:
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either: 129 or CD-1
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
1G Lhx2:
embryonic day 8.25
(more )
Specimen
1G Lhx2:
(close )
Genetic Background:
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either: 129 or CD-1
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Age:
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embryonic day 8.25
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Structure |
Level |
Pattern |
Image |
Note |
TS13: eye neural ectoderm |
Present |
Regionally restricted |
1G Lhx2
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Expression demarcates specific region of anterior neural plate corresponding to presumptive retina field.
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