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Gene Expression Data
Assay Details
Assay
Reference: J:101358 Zekri L, et al., Control of fetal growth and neonatal survival by the RasGAP-associated endoribonuclease G3BP. Mol Cell Biol. 2005 Oct;25(19):8703-16
Assay type: RT-PCR
MGI Accession ID: MGI:6197219
Gene symbol: Igf2
Gene name: insulin-like growth factor 2
Probe: Igf2-pC2, Igf2-pD2
Probe preparation: labelled with SYBR green
Assay notes: Quantitative RT-PCR. Expression in the wild-type was normalized to GAPDH and was set at 1 and compared to levels in -/- embryos.
Results Image: 7A Igf2
 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified Amount Genetic BackgroundMutant Allele(s)Sex
E12.5 +/+ E12.5 TS20: embryo Present 5 µg; total RNA Not Specified Not Specified
E12.5 -/- E12.5 TS20: embryo Present (a) 5 µg; total RNA involves: 129S2/SvPas G3bp1tm1Tazi/G3bp1tm1TaziNot Specified
E13.5 +/+ E13.5 TS21: embryo Present 5 µg; total RNA Not Specified Not Specified
E13.5 -/- E13.5 TS21: embryo Present (b) 5 µg; total RNA involves: 129S2/SvPas G3bp1tm1Tazi/G3bp1tm1TaziNot Specified
E15.5 +/+ E15.5 TS23: embryo Present 5 µg; total RNA Not Specified Not Specified
E15.5 -/- E15.5 TS23: embryo Present (c) 5 µg; total RNA involves: 129S2/SvPas G3bp1tm1Tazi/G3bp1tm1TaziNot Specified
E17.5 +/+ E17.5 TS25: embryo Present 5 µg; total RNA Not Specified Not Specified
E17.5 -/- E17.5 TS25: embryo Present (d) 5 µg; total RNA involves: 129S2/SvPas G3bp1tm1Tazi/G3bp1tm1TaziNot Specified
Notes:
(a) Expression was unexpectedly low in the E12.5 -/- compared to wild type.
(b) More abundant expression was demonstrated at E13.5 in the -/- embryos compared to wild type.
(c) The highest level of expression was detected at E15.5 in the -/- embryos and continued to be more abundant than in wild type.
(d) Expression at E17.5 -/- was comparable to that in the wild type.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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12/30/2025
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