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Gene Expression Data
Assay Details
Assay
Reference: J:142873 Collins CA, et al., Dynamic regulation of retinoic acid-binding proteins in developing, adult and neoplastic skin reveals roles for beta-catenin and Notch signalling. Dev Biol. 2008 Dec 1;324(1):55-67
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6196078
Gene symbol: Crabp1
Gene name: cellular retinoic acid binding protein I
Antibody: anti-CRABP1
Detection system: Alexa Fluor 488
Results
Specimen 1b: embryonic day 13.5 (more )
Note: Double labeled: green - Crabp1; red - Krt14 (assay MGI:6196081).
Structure Level Pattern Image Note
TS21: back skin Present Regionally restricted 1b Expression first seen at E13.5 during earliest stage of skin development, when epidermis consists of single layer of keratinocytes. Expression in cytoplasm of cells with fibroblast-type morphology, closely associated with basal surface.
TS21: epidermis Absent 1b No expression in Krt14-expressing epidermal cells.

Specimen 1c: embryonic day 13.5 (more )
Note: Double labeled: green - Crabp1; red - Mki67 (assay MGI:6196083).
Structure Level Pattern Image Note
TS21: back skin Present Regionally restricted 1c

Specimen 1d: embryonic day 15.5 (more )
Note: Double labeled: green - Crabp1; red - Krt14 (assay MGI:6196081).
Structure Level Pattern Image Note
TS23: hair follicle dermal papilla Strong 1d

Specimen 1e: embryonic day 15.5 (more )
Note: Double labeled: green - Crabp1; red - Mki67 (assay MGI:6196083).
Structure Level Pattern Image Note
TS23: dermis Strong Regionally restricted 1e Strong expression in dermal condensate.

Specimen 1f: embryonic day 17.5 (more )
Note: Double labeled: green - Crabp1; red - Krt14 (assay MGI:6196081).
Structure Level Pattern Image Note
TS25: hair follicle dermal papilla Present Regionally restricted 1f Strong expression in dermal papilla of new secondary hair pegs. Lower intensity of expression in dermal papilla of more mature primary follicles.

Specimen 1g: embryonic day 17.5 (more )
Note: Double labeled: green - Crabp1; red - Mki67 (assay MGI:6196083).
Structure Level Pattern Image Note
TS25: dermis Present Regionally restricted 1g Expression maintained in dermal condensate, in cells concentrated at interface of dermis with epidermis.

Specimen 1h: postnatal day 1 (more )
Note: Double labeled: green - Crabp1; red - Krt14 (assay MGI:6196081).
Structure Level Pattern Image Note
TS27: back skin Weak Regionally restricted 1h
TS27: hair follicle dermal papilla Present 1h Expression remained detectable in all dermal papilla.

Specimen 1i: postnatal day 1 (more )
Note: Double labeled: green - Crabp1; red - Mki67 (assay MGI:6196083).
Structure Level Pattern Image Note
TS27: back skin Weak Regionally restricted 1i Reduced intensity of expression relative to embryonic skin.
TS27: dermis Present Regionally restricted 1i Expression in dermal fibroblasts at dermal-epidermal interface.

Specimen 1j: postnatal day 2 (more )
Note: Double labeled: green - Crabp1; red - Krt14 (assay MGI:6196081).
Structure Level Pattern Image Note
TS27: dermis Weak Regionally restricted 1j Intensity of expression in dermal fibroblasts was lower than at P1.

Specimen 1l: postnatal day 5 (more )
Note: Double labeled: green - Crabp1; red - Krt14 (assay MGI:6196081).
Structure Level Pattern Image Note
TS28: hair follicle dermal papilla Strong Widespread 1l High intensity of expression in dermal papilla of all hair follicles.
TS28: dermis Absent 1l Expression undetectable in dermal fibroblasts.

Specimen 1l inset: postnatal day 5 (more )
Note: Double labeled: green - Crabp1; red - Krt14 (assay MGI:6196081).
Structure Level Pattern Image Note
TS28: back skin Present Regionally restricted 1l inset Expression in small population of cells located beneath the developing sebaceous gland, whose morphology differed from that of surrounding dermal fibroblasts.

Specimen 1n: postnatal day 42 (more )
Note: Double labeled: green - Crabp1; red - Krt14 (assay MGI:6196081).
Structure Level Pattern Image Note
TS28: hair follicle dermal papilla Strong Regionally restricted 1n Strong expression in dermal papilla of telogen (resting) hair follicles.

Specimen 1o: postnatal day 102 (more )
Note: Double labeled: green - Crabp1; red - Krt14 (assay MGI:6196081).
Structure Level Pattern Image Note
TS28: hair follicle dermal papilla Strong Regionally restricted 1o Strong expression in dermal papilla of anagen (growing) hair follicles.


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
05/14/2024
MGI 6.23
The Jackson Laboratory