Antibody:	PCD-1
Detection system:	Secondary antibody coupled to fluorescein

Structure	Level	Pattern	Image	Note
TS21: epidermis	Present		1C	Equal intensity in periderm and epidermis.
TS21: periderm	Present		1C	Equal intensity in periderm and epidermis.

Structure	Level	Pattern	Image	Note
TS22: periderm	Absent		2C
TS22: epidermis	Strong	Regionally restricted	2C	Intense expression in the placodes. Moderate expression in the basal epidermal layer. No expression in the periderm and upper epidermal layer.
TS22: hair epidermal placode	Strong		2C

Structure	Level	Pattern	Image	Note
TS23: periderm	Absent		3C
TS23: epidermis	Present	Regionally restricted	3C	All placodal cells and the cells at the base of each hair plug showed moderate-to-intense staining. The basal epidermal layer stained faintly, while the periderm and upper epidermal layers were negative.
TS23: hair follicle	Present	Regionally restricted	3C	All placodal cells and the cells at the base of each hair plug showed moderate-to-intense staining. The basal epidermal layer stained faintly, while the periderm and upper epidermal layers were negative.
TS23: hair epidermal placode	Present		3C	Moderate-to-intense staining in all placodal cells and the cells at the base of each hair plug.

Structure	Level	Pattern	Image	Note
TS24: hair follicle	Present		4C	Expressed in a tylotrich follicle.
TS24: hair epidermal placode	Strong		4C	Expressed in awl hair plug and awl placode.

Structure	Level	Pattern	Image	Note
TS25: hair epidermal placode	Present		5C	Increased expression in awl hair plugs.
TS25: hair follicle	Present		5C	Increased expression in awl hair plugs and intense expression in the future hair matrix. Faint or moderate expression in the outer follicle epithelial cells, and negative in the core of the follicle cylinder.

Structure	Level	Pattern	Image	Note
TS26: hair follicle	Strong		6C	Expressed in tylotrich follicle, awl follicles and zigzag placodes. Intense staining in the hair matrix of tylotrich and awl follicles. Faint or moderate expression in the outer follicle epithelial cells, and negative in the core of the follicle cylinder.
TS26: hair outer root sheath	Moderate		6C	Faint or moderate expression.
TS26: hair epidermal placode	Present	Regionally restricted	6C	Expressed in the late awl follicles or early zigzag follicles in the basal epidermal layer.

Structure	Level	Pattern	Image	Note
TS27: hair root sheath matrix	Strong
TS27: hair outer root sheath	Moderate