Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:242163
Aavani T, et al., Temporal profiling of photoreceptor lineage gene expression during murine retinal development. Gene Expr Patterns. 2017 Jan;23-24:32-44
|
|
Assay type:
|
RNA in situ
|
|
MGI Accession ID:
|
MGI:6195165
|
|
Gene symbol:
|
Opn1mw
|
|
Gene name:
|
opsin 1 (cone pigments), medium-wave-sensitive (color blindness, deutan)
|
|
Probe:
|
MGC clone 25229
|
|
Probe preparation:
|
Antisense labelled with digoxigenin RNA
|
|
Visualized with:
|
Alkaline phosphatase
|
|
|
Results
|
Specimen
not shown:
embryonic day 12.5
(more  )
Specimen
not shown:
(close  )
|
Genetic Background:
|
CD-1
|
|
Age:
|
embryonic day 12.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
S2C:
embryonic day 15.5
(more )
Specimen
S2C:
(close )
|
Genetic Background:
|
CD-1
|
|
Age:
|
embryonic day 15.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
5A:
postnatal day 0
(more )
Specimen
5A:
(close )
|
Genetic Background:
|
CD-1
|
|
Age:
|
postnatal day 0
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS27: retina outer nuclear layer |
Present |
Regionally restricted |
5A'
,
5A
,
5A''
|
Expression was detected in the upper presumptive outer nuclear layer. Significantly fewer cone photoreceptors expressed Opn1mw in ventral domains of the retina compared to dorsal domains.
|
Specimen
5B:
postnatal day 7
(more )
Specimen
5B:
(close )
|
Genetic Background:
|
CD-1
|
|
Age:
|
postnatal day 7
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS28: retina outer nuclear layer |
Present |
Regionally restricted |
5B'
,
5B
,
5B''
|
Expression was detected in the upper outer nuclear layer. Significantly fewer cone photoreceptors expressed Opn1mw in ventral domains of the retina compared to dorsal domains.
|
Specimen
5C:
postnatal day 14
(more )
Specimen
5C:
(close )
|
Genetic Background:
|
CD-1
|
|
Age:
|
postnatal day 14
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS28: retina outer nuclear layer |
Present |
Regionally restricted |
5C
,
5C''
,
5C'
|
Expression was detected in the upper outer nuclear layer. Significantly fewer cone photoreceptors expressed Opn1mw in ventral domains of the retina compared to dorsal domains.
|
Specimen
5D:
postnatal day 21
(more )
Specimen
5D:
(close )
|
Genetic Background:
|
CD-1
|
|
Age:
|
postnatal day 21
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS28: retina outer nuclear layer |
Present |
Regionally restricted |
5D'
,
5D
,
5D''
|
Expression was detected in the upper outer nuclear layer. Significantly fewer cone photoreceptors expressed Opn1mw in ventral domains of the retina compared to dorsal domains.
|
Specimen
5E:
postnatal day 60
(more )
Specimen
5E:
(close )
|
Genetic Background:
|
CD-1
|
|
Age:
|
postnatal day 60
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS28: retina outer nuclear layer |
Present |
Regionally restricted |
5E
,
5E'
,
5E''
|
Expression was detected in the upper outer nuclear layer. Significantly fewer cone photoreceptors expressed Opn1mw in ventral domains of the retina compared to dorsal domains.
|
|
Analysis Tools