Probe:	Efemp1 cDNA2
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS15: thoracic segment of trunk	Present	Regionally restricted	3A	Expression is in a distinct stripe of cells around the ventral side of the embryo caudal to the developing heart.
TS15: head mesenchyme	Present		3A

Structure	Level	Pattern	Image	Note
TS15: head mesenchyme	Present		3B
TS15: somite	Present		3B
TS15: trunk paraxial mesenchyme	Present		3B	Expression is in the proximal trunk area.

Structure	Level	Pattern	Image	Note
TS15: sclerotome	Present		3C

Structure	Level	Pattern	Image	Note
TS15: sclerotome	Present		3D

Structure	Level	Pattern	Image	Note
TS16: 2nd branchial arch mesenchyme	Present	Regionally restricted	3E	Expression is in mesenchymal cells of the proximal side of first distal side of second branchial arch.
TS16: 3rd branchial arch mesenchyme derived from neural crest	Present		3E
TS16: skeleton	Present	Regionally restricted	3E	Expression is in condensing cells forming the cartilage and bones of the facial skeleton.
TS16: thoracic segment of trunk	Present	Regionally restricted	3E	Expression is in cells of the body wall surrounding the developing heart.

Structure	Level	Pattern	Image	Note
TS16: thoracic segment of trunk	Present	Regionally restricted	3F	Expression is in cells of the body wall surrounding the developing heart.

Structure	Level	Pattern	Image	Note
TS17: vertebral cartilage condensation	Strong		3G
TS17: branchial arch	Present		3G

Structure	Level	Pattern	Image	Note
TS19: vertebral cartilage condensation	Very strong		3H
TS19: skeleton	Present	Regionally restricted	3H	Expression is in regions of developing filamentous bone formation in the craniofacial area.
TS19: cartilage condensation	Present	Regionally restricted	3H	Expression is in cartilage primordia of the limb buds.
TS19: limb mesenchyme	Present	Regionally restricted	3H	Expression is in cartilage primordia of the limb buds.

Structure	Level	Pattern	Image	Note
TS19: vertebral cartilage condensation	Very strong		3I

Structure	Level	Pattern	Image	Note
TS20: vertebral cartilage condensation	Present		3K

Structure	Level	Pattern	Image	Note
TS22: cranium bone primordium	Present	Regionally restricted	4A	Expression is in the turbinate bone.
TS22: chondrocranium bone	Present	Regionally restricted	4A	Expression is in the basisphenoid.
TS22: C1 vertebra	Present		4A
TS22: hyoid cartilage condensation	Present		4A
TS22: rib cartilage condensation	Present		4A
TS22: vertebra	Present		4A

Structure	Level	Pattern	Image	Note
TS22: temporal bone	Present		4B

Structure	Level	Pattern	Image	Note
TS23: rib	Present		4C
TS23: vertebra	Present		4C

Structure	Level	Pattern	Image	Note
TS23: forelimb bone	Present		4D

Structure	Level	Pattern	Image	Note
TS21: eye	Absent		4E

Structure	Level	Pattern	Image	Note
TS23: eye	Absent		4F

Structure	Level	Pattern	Image	Note
TS21: forelimb cartilage condensation	Present
TS21: hindlimb cartilage condensation	Present
TS21: skeleton	Present	Regionally restricted		Expression is in areas of ossification of the membraneous bones of the facial skeleton.