Antibody:	anti-beta-catenin (C-18): sc-1496
Detection system:	horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS21: cap mesenchyme	Present		1A	Expression localized to stalks and tips of ureteric bud, nephrogenic structures, and uninduced metanephric mesenchyme.
TS21: pretubular aggregate	Present		1A	Expression localized to stalks and tips of ureteric bud, nephrogenic structures, and uninduced metanephric mesenchyme.
TS21: ureteric tip	Present		1A	Expression localized to stalks and tips of ureteric bud, nephrogenic structures, and uninduced metanephric mesenchyme.
TS21: ureteric tree terminal branch excluding tip itself	Present		1A	Expression localized to stalks and tips of ureteric bud, nephrogenic structures, and uninduced metanephric mesenchyme.

Structure	Level	Pattern	Image	Note
TS23: cap mesenchyme	Present		1B	Authors report a similar pattern of expression to that seen at E13.5. Expression localized to both ureteric bud branches and nephrogenic intermediate structures in a pattern consistent with expression in adherens junctions.
TS23: pretubular aggregate	Present		1B	Authors report a similar pattern of expression to that seen at E13.5. Expression localized to both ureteric bud branches and nephrogenic intermediate structures in a pattern consistent with expression in adherens junctions.
TS23: ureteric tip	Present		1B	Authors report a similar pattern of expression to that seen at E13.5. Expression localized to both ureteric bud branches and nephrogenic intermediate structures in a pattern consistent with expression in adherens junctions.
TS23: ureteric tree terminal branch excluding tip itself	Present		1B	Authors report a similar pattern of expression to that seen at E13.5. Expression localized to both ureteric bud branches and nephrogenic intermediate structures in a pattern consistent with expression in adherens junctions.

Structure	Level	Pattern	Image	Note
TS21: cap mesenchyme	Present		1E	Expression in ureteric bud and metanephric mesenchyme
TS21: ureteric tip	Present		1E	Expression in ureteric bud and metanephric mesenchyme

Structure	Level	Pattern	Image	Note
TS21: collecting duct	Absent		1F	No expression in ureteric bud.
TS21: cap mesenchyme	Present		1F	Expression maintained in metanephric mesenchyme.