Gene Expression Data
Assay Details
Assay
|
Reference:
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J:226319
Phippard D, et al., Changes in the subcellular localization of the Brn4 gene product precede mesenchymal remodeling of the otic capsule. Hear Res. 1998 Jun;120(1-2):77-85
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:6193753
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Gene symbol:
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Pou3f4
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Gene name:
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POU domain, class 3, transcription factor 4
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Probe:
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Brn4 cDNA2
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Probe preparation:
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Antisense labelled with S35 RNA
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Visualized with:
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Autoradiography
|
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Results
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Specimen
not shown:
embryonic day 9.5
(more )
Specimen
not shown:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 9.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
1A/B:
embryonic day 10.5
(more )
Specimen
1A/B:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 10.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: otocyst mesenchyme |
Present |
Regionally restricted |
1A/B
|
Expression was detected predominantly in the more extensively condensed mesenchyme of the ventral half of the otocyst. Low levels of expression were detected in the aggregation center in the dorsomedial aspect.
|
TS17: branchial arch mesenchyme |
Present |
|
1A/B
|
|
TS17: hindbrain |
Present |
|
1A/B
|
|
Specimen
2A/B:
embryonic day 11.5
(more )
Specimen
2A/B:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: otocyst |
Present |
Regionally restricted |
2A/B
|
Expression was even more pronounced in the mesenchymal condensation.
|
Specimen
3A/B:
embryonic day 14.5
(more )
Specimen
3A/B:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
3C/D:
embryonic day 14.5
(more )
Specimen
3C/D:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: otic capsule |
Present |
Widespread |
3C/D
|
Expression was detected in both the cochlear and vestibular components of the otic capsule.
|
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