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Gene Expression Data
Assay Details
Assay
Reference: J:260419 Jiang X, et al., Asymmetric distribution of CRUMBS polarity complex proteins from compacted 8-cell to blastocyst stage during mouse preimplantation development. Gene Expr Patterns. 2018 Jan;27:93-98
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6192281
Gene symbol: Patj
Gene name: PATJ, crumbs cell polarity complex component
Antibody: anti-PATJ (GTX104753)
Detection system: Secondary antibody coupled to fluorescein
Assay notes: Confocal images.
Results
Specimen 4 c8c: embryonic day 2.5 (more )
Note: Compacted 8-cell embryo.
Structure Level Pattern Image Note
TS3: 8-cell stage embryo Present Regionally restricted 4_c8c , 4_c8c Merge Expression is at tight junctions of outer blastomere contacts and at the apical membrane adjacent to tight junctions.
Specimen 4 Mo: embryonic day 3.0 (more )
Note: Morula stage embryo.
Structure Level Pattern Image Note
TS3: compacted morula Present Regionally restricted 4_Mo , 4_Mo Merge Expression is at tight junctions of outer blastomere contacts and at the apical membrane adjacent to tight junctions. Very weak expression is found at the basolateral membrane.
Specimen 4 Bl: embryonic day 3.5 (more )
Note: Blastocyst stage embryo.
Structure Level Pattern Image Note
TS4: inner cell mass Present Regionally restricted 4_Bl Merge , 4_Bl Expression is at tight junctions of outer blastomere contacts and at the apical membrane adjacent to tight junctions. Very weak expression is found at the basolateral membrane.
Specimen 6C: embryonic day 3.5 (more )
Note: Blastocyst stage embryo. Double labeled: green - Patj; red - Tjp1 (assay MGI:6192291).
Structure Level Pattern Image Note
TS4: trophectoderm Present Regionally restricted 6C Merge , 6C PATJ Expression is at tight junctions in the outer trophectoderm cells.
TS4: inner cell mass Present Regionally restricted 6C Merge , 6C PATJ Expression is at the apical membrane adjacent to tight junctions.
Specimen S4 Zy: embryonic day 0.5 (more )
Note: Zygote.
Structure Level Pattern Image Note
TS1: 1-cell stage embryo Weak Uniform S4_Zy Merge , S4_Zy Expression is in the cytoplasm.
Specimen S4 2c: embryonic day 1.0 (more )
Note: 2-cell stage embryo.
Structure Level Pattern Image Note
TS2: 2-cell stage embryo Weak Uniform S4_2c , S4_2c Merge Expression is in the cytoplasm.
Specimen S4 4c: embryonic day 2.0 (more )
Note: 4-cell stage embryo.
Structure Level Pattern Image Note
TS3: 4-cell stage embryo Weak Uniform S4_4c Merge , S4_4c Expression is in the cytoplasm.
Specimen S4 8c: embryonic day 2.0 (more )
Note: 8-cell stage embryo.
Structure Level Pattern Image Note
TS3: 8-cell stage embryo Weak Uniform S4_8c Merge , S4_8c Expression is in the cytoplasm.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/21/2024
MGI 6.23 		The Jackson Laboratory