Antibody:	anti-Tuj1
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
Assay notes:	Fixation was Bouin's or 8% paraformaldehyde.

Structure	Level	Pattern	Image	Note
TS24: superior cervical ganglion	Present		1B
TS24: glossopharyngeal nerve	Present	Regionally restricted	1B	Baroreceptors of the glossopharyngeal nerve are distributed in the proximal portion (carotid sinus) at root of the internal carotid artery.

Structure	Level	Pattern	Image	Note
TS26: superior cervical ganglion	Present		3B
TS26: internal carotid artery	Absent		3B	Authors report innervation of baroreceptor was not localized to the root of the internal carotid artery.

Structure	Level	Pattern	Image	Note
TS21: inferior cervical ganglion	Present		5B	Authors report that the superior cervical ganglion is separated from the stellate ganglion.
TS21: superior cervical ganglion	Present		5B	Authors report that the superior cervical ganglion is separated from the stellate ganglion.

Structure	Level	Pattern	Image	Note
TS20: glossopharyngeal nerve	Present	Regionally restricted	6A	Nerve fibers issuing from the glossopharyngeal nerve are distributed around the third arch artery.

Structure	Level	Pattern	Image	Note
TS20: glossopharyngeal nerve	Weak	Regionally restricted	6B	In mutant embryo, few fibers are detected around the artery.

Structure	Level	Pattern	Image	Note
TS21: superior cervical ganglion	Present		6C
TS21: carotid artery	Present	Regionally restricted	6C	Authors state neuronal cells begin to enter carotid body rudiment, in which nerve fibers were densely distributed. Carotid sinus nerve and sympathetic nerve fibers were intermingled with the carotid body rudiment.

Structure	Level	Pattern	Image	Note
TS21: superior cervical ganglion	Present	Regionally restricted	6D	Authors state the superior cervical ganglion failed to extend process around the third arch artery.
TS21: carotid artery	Absent		6D	Carotid body rudiment was not formed in the wall of the third arch artery.

Structure	Level	Pattern	Image	Note
TS21: superior cervical ganglion	Present		7B
TS21: carotid artery	Present	Regionally restricted	7B	Authors state neuronal cells begin to enter carotid body rudiment, in which nerve fibers were densely distributed. Carotid sinus nerve and sympathetic nerve fibers were intermingled with the carotid body rudiment.

Structure	Level	Pattern	Image	Note
TS21: superior cervical ganglion	Present	Regionally restricted	7D	Authors state the superior cervical ganglion failed to extend process around the third arch artery.
TS21: carotid artery	Absent		7D	Carotid body rudiment was not formed in the wall of the third arch artery.

Structure	Level	Pattern	Image	Note
TS23: superior cervical ganglion	Present		8A
TS23: carotid artery	Present	Regionally restricted	8A	Authors report baroreceptors were densely distributed at the root of the internal carotid artery.

Structure	Level	Pattern	Image	Note
TS23: superior cervical ganglion	Present		8B
TS23: carotid artery	Absent		8B	Neither carotid body nor baroreceptor fibers were detected in the carotid bifurcation of mutatnt embryos.