Antibody:	anti-Dlx-2
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS17: 1st branchial arch mandibular component ectoderm	Present	Regionally restricted	1	Expression is at the anterior end.
TS17: 1st branchial arch maxillary component ectoderm	Present	Regionally restricted	1	Expression is at the anterior end.
TS17: 1st branchial arch mandibular component mesenchyme	Present	Regionally restricted	1	Expression is in the mesenchyme underlying the non-expressing ectoderm to the posterior of the arch.
TS17: 1st branchial arch maxillary component mesenchyme	Present	Regionally restricted	1	Expression is in the mesenchyme underlying the non-expressing ectoderm to the posterior of the arch.

Structure	Level	Pattern	Image	Note
TS20: oral epithelium	Present	Regionally restricted	2A	Expression is in the basal cells of the invaginating epithelia of the tooth germs within the first branchial arch. Expression is strongest at the base of the tooth germ.
TS20: tooth bud	Present	Regionally restricted	2A	Expression is in the basal cells of the invaginating epithelia of the tooth germs within the first branchial arch. Expression is strongest at the base of the tooth germ.

Structure	Level	Pattern	Image	Note
TS21: lower jaw mesenchyme	Present	Regionally restricted	2B	Expression is in the mesenchyme adjacent to the tooth germ and is initially seen at the buccal aspect and becomes stronger around the base of the tooth germ.

Structure	Level	Pattern	Image	Note
TS22: lower jaw mesenchyme	Strong	Regionally restricted	2C	Expression is in mesenchymal cells condensing around the base of the toothbud.
TS22: lower jaw tooth epithelium	Present		2C
TS22: oral epithelium	Present	Regionally restricted	2C	Expression is in the vestibular lamina.

Structure	Level	Pattern	Image	Note
TS23: upper jaw tooth dental papilla	Strong	Regionally restricted	3A	Expression is strongest adjacent to the internal enamel epithelium.
TS23: inner enamel epithelium	Weak		3A
TS23: outer enamel epithelium	Weak		3A
TS23: stellate reticulum	Weak		3A
TS23: stratum intermedium	Weak		3A
TS23: upper jaw tooth enamel organ	Weak		3A
TS23: cap stage tooth	Present		3A

Structure	Level	Pattern	Image	Note
TS23: lower jaw tooth dental papilla	Strong	Regionally restricted	3B	Expression is strongest adjacent to the internal enamel epithelium.
TS23: inner enamel epithelium	Weak		3B
TS23: lower jaw tooth enamel organ	Weak		3B
TS23: outer enamel epithelium	Weak		3B
TS23: stellate reticulum	Weak		3B
TS23: stratum intermedium	Weak		3B
TS23: cap stage tooth	Present		3B

Structure	Level	Pattern	Image	Note
TS25: lower jaw incisor enamel organ	Strong	Regionally restricted	4	Expression is in the differentiated ameloblasts.
TS25: outer enamel epithelium	Strong		4
TS25: stellate reticulum	Strong		4
TS25: stratum intermedium	Strong		4
TS25: lower jaw incisor dental papilla	Present		4
TS25: bell stage tooth	Present		4

Structure	Level	Pattern	Image	Note
TS25: upper jaw incisor enamel organ	Strong	Regionally restricted	5A	Expression is in strongest in ameloblasts.
TS25: stratum intermedium	Strong		5A
TS25: upper jaw incisor dental papilla	Present		5A
TS25: bell stage tooth	Present		5A

Structure	Level	Pattern	Image	Note
TS25: upper jaw incisor enamel organ	Strong	Regionally restricted	5B	Expression is in strongest in ameloblasts.
TS25: stratum intermedium	Strong		5B
TS25: upper jaw incisor dental papilla	Present		5B
TS25: bell stage tooth	Present		5B

Structure	Level	Pattern	Image	Note
TS26: enamel organ	Strong		6	Expression is strongest in ameloblasts.
TS26: dental papilla	Weak		6
TS26: late bell stage tooth	Present		6

Structure	Level	Pattern	Image	Note
TS24: dental papilla	Present	Regionally restricted		Expression is strongest adjacent to the internal enamel epithelium.
TS24: outer enamel epithelium	Weak
TS24: stellate reticulum	Weak
TS24: stratum intermedium	Weak
TS24: early bell stage tooth	Present