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Gene Expression Data
Assay Details
Assay
Reference: J:128009 Wirrig EE, et al., Cartilage link protein 1 (Crtl1), an extracellular matrix component playing an important role in heart development. Dev Biol. 2007 Oct 15;310(2):291-303
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6163729
Gene symbol: Vcan
Gene name: versican
Antibody: anti-mouse versican (GAG-beta domain) (AB1033)
Detection system: Alexa Fluor 568
Results
Specimen 2B: embryonic day 9.5 (more )
Structure Level Pattern Image Note
TS15: atrioventricular endocardial cushion Present 2B
Specimen 2C: embryonic day 9.5 (more )
Note: Double labeled: red - Vcan; green - Crtl1 (Hapln1 assay MGI:6163728).
Structure Level Pattern Image Note
TS15: heart Present Regionally restricted 2C Authors report high degree of co-expression with Crtl1.
Specimen 2E: embryonic day 9.5 (more )
Structure Level Pattern Image Note
TS15: atrioventricular endocardial cushion Present Regionally restricted 2E Expression in extracellular matrix surrounding endocardial lining of ventricular trabeculae.
Specimen 2F: embryonic day 9.5 (more )
Note: Double labeled: red - Vcan; green - Crtl1 (Hapln1 assay MGI:6163728).
Structure Level Pattern Image Note
TS15: atrioventricular endocardial cushion Present Regionally restricted 2F Expression in extracellular matrix surrounding endocardial lining of ventricular trabeculae.
Specimen 2G: embryonic day 10.5 (more )
Note: Double labeled: red - Vcan; green - Crtl1 (Hapln1 assay MGI:6163728).
Structure Level Pattern Image Note
TS17: outflow tract endocardial cushion Strong Regionally restricted 2G Abundant expression in sub-endocardial matrix of conal cushions in proximal outflow tract.
TS17: truncus arteriosus Present Regionally restricted 2G Expression in sub-endocardial mesenchyme of truncal outflow tract ridges.
Specimen 2H: embryonic day 10.5 (more )
Note: Double labeled: red - Vcan; green - Crtl1 (Hapln1 assay MGI:6163728).
Structure Level Pattern Image Note
TS17: atrioventricular endocardial cushion Present 2H Authors report high degree of co-expression with Ctrl1 in atrioventricular cushions.
Specimen 3C: embryonic day 13.0 (more )
Structure Level Pattern Image Note
TS21: outflow tract Present Regionally restricted 3C Expression in all components of conal and truncal ridges. Authors report expression overlapping with Ctrl1 and hyaluronan in conal cushions. Authors report expression in parts of truncal ridges were Ctrl1 expression is absent.
TS21: endocardial cushion Strong Regionally restricted 3C Most prominent expression in endocardially-derived mesenchyme.
TS21: semilunar valve Present 3C Authors report expression overlapping with Ctrl1 and hyaluronan in semilunar valves.
Specimen 3G: embryonic day 13.0 (more )
Structure Level Pattern Image Note
TS21: heart mesenchyme Present Regionally restricted 3G Authors report lower levels of expression dorsal mesenchymal protrusion than in surrounding mesenchyme.
Specimen 4B/C: embryonic day 14.5 (more )
Note: Double labeled: red - Vcan; green - Crtl1 (Hapln1 assay MGI:6163728).
Structure Level Pattern Image Note
TS22: tricuspid valve Strong 4B , 4C Authors report overlapping expression with Ctrl1 in leaflets of tricuspid valve. Higher expression in tricuspid valve leaflets compared to leaflets of mitral valve.
TS22: mitral valve Present 4C , 4B Authors report overlapping expression with Ctrl1 in leaflets of mitral atrioventricular valvs.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory