Antibody:	anti-P450c17
Detection system:	Secondary antibody coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS16: dorsal root ganglion	Present			Expression was in both cell bodies and fibers.
TS16: neural crest	Present			Expression was in both cell bodies and fibers.
TS16: peripheral nervous system	Present			Expression was in both cell bodies and fibers of the sympathetic ganglia.

Structure	Level	Pattern	Image	Note
TS20: dorsal root ganglion	Present			Expression was in both cell bodies and fibers.
TS20: spinal cord	Present			Expression was in both cell bodies and fibers.
TS20: sympathetic ganglion	Present			Expression was in both cell bodies and fibers.
TS20: trigeminal ganglion	Present			Expression was in both cell bodies and fibers.

Structure	Level	Pattern	Image	Note
TS24: cerebellum	Present			Expression was detected in fibers.
TS24: cerebral internal capsule	Present			Expression was detected in fibers.
TS24: diaphragm	Present			Expression was detected in fibers.
TS24: heart	Present			Expression was detected in fibers.
TS24: intestine	Present			Expression was detected in fibers.
TS24: pons	Present			Expression was detected in fibers.
TS24: tongue	Present			Expression was detected in fibers.
TS24: vibrissa follicle	Present			Expression was detected in fibers.
TS24: brainstem nucleus	Present			Expression was detected in cell bodies in the pontine nucleus.
TS24: dorsal root ganglion	Present			Expression was in both cell bodies and fibers.
TS24: medulla oblongata	Present			Expression was in both cell bodies and fibers.
TS24: sympathetic ganglion	Present			Expression was in both cell bodies and fibers.
TS24: trigeminal ganglion	Present			Expression was in both cell bodies and fibers.
TS24: spinal cord	Present			Expression was detected in fibers in the dorsal spinal cord. Expression was detected in cell bodies in the ventral spinal cord.

Structure	Level	Pattern	Image	Note
TS26: cerebellum	Present			Expression was detected in fibers.
TS26: cerebral internal capsule	Present			Expression was detected in fibers.
TS26: diaphragm	Present			Expression was detected in fibers.
TS26: heart	Present			Expression was detected in fibers.
TS26: hippocampus	Present			Expression was detected in fibers.
TS26: hypoglossal nerve	Present			Expression was detected in fibers.
TS26: intestine	Present			Expression was detected in fibers.
TS26: neocortex	Present			Expression was detected in fibers.
TS26: pons	Present			Expression was detected in fibers.
TS26: tectum	Present			Expression was detected in fibers.
TS26: tongue	Present			Expression was detected in fibers.
TS26: vibrissa follicle	Present			Expression was detected in fibers.
TS26: celiac ganglion	Present			Expression was in both cell bodies and fibers.
TS26: cochlear ganglion	Present			Expression was in both cell bodies and fibers.
TS26: dorsal root ganglion	Present			Expression was in both cell bodies and fibers.
TS26: medulla oblongata	Present			Expression was in both cell bodies and fibers.
TS26: midbrain	Present			Expression was in both cell bodies and fibers.
TS26: sympathetic ganglion	Present			Expression was in both cell bodies and fibers.
TS26: trigeminal ganglion	Present			Expression was in both cell bodies and fibers.
TS26: spinal cord	Present			Expression was detected in fibers in the dorsal spinal cord. Expression was detected in cell bodies in the ventral spinal cord.
TS26: hippocampal formation	Present	Regionally restricted		Expression was detected in fibers in the subicular area.
TS26: brainstem nucleus	Present	Regionally restricted		Expression was detected in cell bodies in the pontine nucleus and trigeminal motor nucleus.
TS26: ganglion	Present	Regionally restricted		Expression was in both cell bodies and fibers of the hypopharyngial ganglia.
TS26: glossopharyngeal nerve	Present	Regionally restricted		Expression was in cell bodies in the nodose-petrosal nerve.
TS26: vagus nerve	Present	Regionally restricted		Expression was in cell bodies in the nodose-petrosal nerve.
TS26: testis	Present			Expression was detected in cell bodies.

Structure	Level	Pattern	Image	Note
TS27: bed nucleus of stria terminalis	Present			Expression was detected in fibers.
TS27: cerebellum	Present			Expression was detected in fibers.
TS27: cerebral internal capsule	Present			Expression was detected in fibers.
TS27: hippocampal commissure	Present			Expression was detected in fibers.
TS27: midbrain	Present			Expression was detected in fibers.
TS27: olfactory tract	Present			Expression was detected in fibers.
TS27: thalamus	Present			Expression was detected in fibers.
TS27: testis	Present			Expression was detected in cell bodies.
TS27: trigeminal V motor nucleus	Present			Expression was detected in cell bodies.
TS27: dorsal root ganglion	Present			Expression was in both cell bodies and fibers.

Structure	Level	Pattern	Image	Note
TS28: bed nucleus of stria terminalis	Present			Expression was detected in fibers.
TS28: cerebellum	Present			Expression was detected in fibers.
TS28: cerebral internal capsule	Present			Expression was detected in fibers.
TS28: hippocampal commissure	Present			Expression was detected in fibers.
TS28: midbrain	Present			Expression was detected in fibers.
TS28: olfactory tract	Present			Expression was detected in fibers.
TS28: thalamus	Present			Expression was detected in fibers.
TS28: testis	Present			Expression was detected in cell bodies.
TS28: trigeminal V motor nucleus	Present			Expression was detected in cell bodies.
TS28: dorsal root ganglion	Present			Expression was in both cell bodies and fibers.

Structure	Level	Pattern	Image	Note
TS26: tongue	Present		3A

Structure	Level	Pattern	Image	Note
TS26: retina nerve fiber layer	Present		3B
TS26: retina inner nuclear layer	Present		3B	Staining of small multipolar neurons in the amacrine cell layer was noted.

Structure	Level	Pattern	Image	Note
TS26: retina inner nuclear layer	Present		3C
TS26: retina nerve fiber layer	Present		3C

Structure	Level	Pattern	Image	Note
TS26: trigeminal ganglion	Present		3D

Structure	Level	Pattern	Image	Note
TS26: pons	Present		3E	Staining was noted in large, multipolar branched neurons. Cytoplasmic staining was juxtamembranous.

Structure	Level	Pattern	Image	Note
TS26: spinal cord	Present	Regionally restricted	3F	Expression in the ventral part of the spinal cord.

Structure	Level	Pattern	Image	Note
TS26: spinal cord	Present	Regionally restricted	3G	Expression in the dorsal part of the spinal cord.

Structure	Level	Pattern	Image	Note
TS26: dorsal root ganglion	Present		3H

Structure	Level	Pattern	Image	Note
TS26: dorsal root ganglion	Present		3I

Structure	Level	Pattern	Image	Note
TS26: dorsal root ganglion	Present		3J

Structure	Level	Pattern	Image	Note
TS26: dorsal root ganglion	Present		3K

Structure	Level	Pattern	Image	Note
TS26: cochlear ganglion	Present		4A	Expression was in cell bodies. Axons deriving from these structures and entering the brain were immunopositive.
TS26: facial ganglion	Present		4A	Expression was in cell bodies. Axons deriving from these structures and entering the brain were immunopositive.
TS26: glossopharyngeal ganglion	Present		4A	Expression was in cell bodies. Axons deriving from these structures and entering the brain were immunopositive.
TS26: trigeminal ganglion	Present		4A	Expression was in cell bodies. Axons deriving from these structures and entering the brain were immunopositive.
TS26: glossopharyngeal nerve	Present		4A	Expression was detected in fibers.
TS26: vagus nerve	Present		4A	Expression was detected in fibers.
TS26: vestibular component epithelium	Present	Regionally restricted	4A	Expression was detected in the macular and ampullar organs of the vestibular sensory epithelium.
TS26: cerebral cortex intermediate zone	Present		4B , 4A
TS26: cortical plate	Present	Regionally restricted	4G , 4B , 4A	Expression was in the deep layers of the cortical plate.
TS26: hippocampal formation	Present	Regionally restricted	4B , 4A	Immunopositive fibers were noted in the region of the subiculum/entorhinal cortex that extended in a superficial and periventricular path into the hippocampus.

Structure	Level	Pattern	Image	Note
TS26: sensory ganglion	Present		4C	Immunopositive cells were mainly large, clear, and unipolar neurons.

Structure	Level	Pattern	Image	Note
TS26: cranial nerve	Present		4D	Axons deriving from cranial sensory ganglia were immunopositive.
TS26: vestibular component epithelium	Present	Regionally restricted	4D	Expression was detected in the macular and ampullar organs of the vestibular sensory epithelium.

Structure	Level	Pattern	Image	Note
TS26: cortical subplate	Present		4E	Expression was found in fibers surrounding cell bodies.

Structure	Level	Pattern	Image	Note
TS24: cerebral internal capsule	Present		4F	Expression was detected in fibers.

Structure	Level	Pattern	Image	Note
TS26: cerebral internal capsule	Present	Regionally restricted	4H	Expression in internal capsule fibers as they penetrate the striatum and enter the cortex.

Structure	Level	Pattern	Image	Note
TS26: vibrissa follicle	Present		5A

Structure	Level	Pattern	Image	Note
TS26: midbrain	Present	Regionally restricted	5C , 5D	Expression was detected in a mesencephalic nucleus.
TS26: deep cerebellar nucleus	Strong		5D , 5C	Strong immunostaining was noted in both fibers and cell bodies.

Structure	Level	Pattern	Image	Note
TS26: medulla oblongata	Present		5E
TS26: deep cerebellar nucleus	Strong		5E	Strong immunostaining was noted in both fibers and cell bodies.

Structure	Level	Pattern	Image	Note
TS26: spinal cord	Present	Regionally restricted	5F	Immunopositive cell bodies are numerous, large, multipolar, and located in the ventral part of the spinal cord, suggesting they are motorneurons.

Structure	Level	Pattern	Image	Note
TS26: brainstem nucleus	Present	Regionally restricted	5G	Expression was detected in the pontine nucleus.

Structure	Level	Pattern	Image	Note
TS28: dorsal root ganglion	Present	Regionally restricted		Immunopositive bipolar cell bodies and axons were observed. Immunopositive cells represent 35% of all cells.

Structure	Level	Pattern	Image	Note
TS28: dorsal root ganglion	Present	Regionally restricted		Cyp17a1 immunostaining didn't overlap with Calca (CGRP)-immunopositive cells.

Structure	Level	Pattern	Image	Note
TS28: dorsal root ganglion	Present	Regionally restricted		All Cyp17a1-immunopositive cells were also immunopositive for Th.

Structure	Level	Pattern	Image	Note
TS28: dorsal root ganglion	Present	Regionally restricted		Less than 10% of the Cyp17a1-immunopositive cells were also Ngfr-immunopositive.

Structure	Level	Pattern	Image	Note
TS28: dorsal root ganglion	Present	Regionally restricted		About 1/5th of the Cyp17a1-immunopositive cells were also Ntrk1-immunopositive.

Structure	Level	Pattern	Image	Note
TS26: central nervous system	Present			There was not overlap of immunostaining with Gfap.

Structure	Level	Pattern	Image	Note
TS26: trigeminal ganglion	Present			Most of the Eno2 immunopositive cells in the trigeminal ganglion were also immunopositive for Cyp17a1.

Structure	Level	Pattern	Image	Note
TS26: brainstem nucleus	Present	Regionally restricted	8A	Although, expression was detected in the position of the locus coeruleus, the staining defined a subset of cells distinct from Dbh-immunostained cells known to characterize the nucleus.