Gene Expression Data
Assay Details
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Assay
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Reference:
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J:253966
Sigg MA, et al., Evolutionary Proteomics Uncovers Ancient Associations of Cilia with Signaling Pathways. Dev Cell. 2017 Dec 18;43(6):744-762.e11
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:6156675
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Gene symbol:
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Lefty2
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Gene name:
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left-right determination factor 2
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Probe:
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Lefty-2 cDNA1
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
6I Enkur+/- top:
embryonic day 8.0; Enkurtm1Hmf/Enkur +
(more  )
Note: 3-5 somite stage embryo.
Specimen
6I Enkur+/- top:
(close  )
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Genetic Background:
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involves: 129 * C57BL/6J
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Mutant Allele(s):
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Enkurtm1Hmf/Enkur+
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Age:
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embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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3-5 somite stage embryo.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: lateral plate mesoderm |
Present |
Regionally restricted |
6I Enkur+/- top
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Expression is in the left lateral plate mesoderm.
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| TS12: embryo |
Present |
Regionally restricted |
6I Enkur+/- top
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Expression is at the midline.
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Specimen
6I Enkur-/- top left:
embryonic day 8.0; Enkurtm1Hmf/ Enkurtm1Hmf
(more )
Note: 3-5 somite stage embryo.
Specimen
6I Enkur-/- top left:
(close )
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Genetic Background:
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involves: 129 * C57BL/6J
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Mutant Allele(s):
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Enkurtm1Hmf/Enkurtm1Hmf
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Age:
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embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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3-5 somite stage embryo.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS12: lateral plate mesoderm |
Present |
Regionally restricted |
6I Enkur-/- top left
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Expression is bilateral and variably enriched on either the left or right side (shown).
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Specimen
6I Enkur-/- top right:
embryonic day 8.0; Enkurtm1Hmf/ Enkurtm1Hmf
(more )
Note: 3-5 somite stage embryo.
Specimen
6I Enkur-/- top right:
(close )
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Genetic Background:
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involves: 129 * C57BL/6J
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Mutant Allele(s):
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Enkurtm1Hmf/Enkurtm1Hmf
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Age:
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embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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|
Note:
|
3-5 somite stage embryo.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS12: lateral plate mesoderm |
Present |
Regionally restricted |
6I Enkur-/- top right
|
Expression is bilateral and variably enriched on either the left (shown) or right side.
|
Specimen
6I Enkur+/- bottom:
embryonic day 8.0; Enkurtm1Hmf/Enkur +
(more )
Note: 3-5 somite stage embryo.
Specimen
6I Enkur+/- bottom:
(close )
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Genetic Background:
|
involves: 129 * C57BL/6J
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Mutant Allele(s):
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Enkurtm1Hmf/Enkur+
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Age:
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embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
|
4% Paraformaldehyde
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|
Note:
|
3-5 somite stage embryo.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS12: lateral plate mesoderm |
Present |
Regionally restricted |
6I Enkur+/- bottom
|
Expression is in the left lateral plate mesoderm.
|
| TS12: embryo |
Present |
Regionally restricted |
6I Enkur+/- bottom
|
Expression is at the midline.
|
Specimen
6I Enkur-/- bottom left:
embryonic day 8.0; Enkurtm1Hmf/ Enkurtm1Hmf
(more )
Note: 3-5 somite stage embryo.
Specimen
6I Enkur-/- bottom left:
(close )
|
Genetic Background:
|
involves: 129 * C57BL/6J
|
|
Mutant Allele(s):
|
Enkurtm1Hmf/Enkurtm1Hmf
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Age:
|
embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
3-5 somite stage embryo.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS12: lateral plate mesoderm |
Present |
Regionally restricted |
6I Enkur-/- bottom left
|
Expression is bilateral and variably enriched on either the left or right side (shown).
|
Specimen
6I Enkur-/- bottom right:
embryonic day 8.0; Enkurtm1Hmf/ Enkurtm1Hmf
(more )
Note: 3-5 somite stage embryo.
Specimen
6I Enkur-/- bottom right:
(close )
|
Genetic Background:
|
involves: 129 * C57BL/6J
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|
Mutant Allele(s):
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Enkurtm1Hmf/Enkurtm1Hmf
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Age:
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embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
|
3-5 somite stage embryo.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS12: lateral plate mesoderm |
Present |
Regionally restricted |
6I Enkur-/- bottom right
|
Expression is bilateral and variably enriched on either the left (shown) or right side.
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