Gene Expression Data
Assay Details
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Assay
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Reference:
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J:238219
Fabrizius A, et al., Critical re-evaluation of neuroglobin expression reveals conserved patterns among mammals. Neuroscience. 2016 Nov 19;337:339-354
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Assay type:
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RT-PCR
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MGI Accession ID:
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MGI:6156304
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Gene symbol:
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Ngb
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Gene name:
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neuroglobin
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Probe:
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Ngb-qPCR-Reg-For, Ngb-qPCR-Reg-Rev
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Probe preparation:
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labelled with SYBR green
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Assay notes:
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Two set of primers were described in the methods: MGI:6156293 and MGI:6156295. The publication didn't specify which was used in this assay. Quantitative RT-PCR. Assay performance was calculated by a serial dilution of a standard-plasmid containing the amplicon sequence. Transcript was quantified using the absolute quantification method with serial dilutions of a known standard, and copy numbers per 1 ug RNA were calculated.
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Results
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Image: 2B
Notes:
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Analysis Tools
