Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:221192
Prasitsak T, et al., Foxc1 is required for early stage telencephalic vascular development. Dev Dyn. 2015 May;244(5):703-11
|
|
Assay type:
|
Immunohistochemistry
|
|
MGI Accession ID:
|
MGI:6154603
|
|
Gene symbol:
|
Pecam1
|
|
Gene name:
|
platelet/endothelial cell adhesion molecule 1
|
|
Antibody:
|
anti-CD31
|
|
Detection system:
|
Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
|
|
|
Results
|
Specimen
2A:
embryonic day 11.0
(more  )
Specimen
2A:
(close  )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 11.0
|
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
2% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS18: brain blood vessel |
Present |
Regionally restricted |
2A
|
Authors report connected vascular networks.
|
Specimen
2B:
embryonic day 11.0
(more )
Specimen
2B:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 11.0
|
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
2% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS18: brain blood vessel |
Present |
Regionally restricted |
2B
|
Top view of medial ganglionic eminence shows formation of a patterned network.
|
Specimen
2C:
embryonic day 11.0
(more )
Specimen
2C:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 11.0
|
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
2% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS18: brain blood vessel |
Present |
Regionally restricted |
2C
|
Side view shows formation of subventricular vascular plexus.
|
Specimen
2D:
(close )
|
Genetic Background:
|
involves: C57BL/6 * CHMU/Le
|
|
Mutant Allele(s):
|
Foxc1ch/Foxc1ch
|
|
Age:
|
embryonic day 11.0
|
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
2% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS18: brain blood vessel |
Present |
Regionally restricted |
2D
|
Authors state overall vascular patterning was normal, although subventricular vascular plexus formation was clearly impaired in teh medial ganglionic eminence.
|
Specimen
2E:
(close )
|
Genetic Background:
|
involves: C57BL/6 * CHMU/Le
|
|
Mutant Allele(s):
|
Foxc1ch/Foxc1ch
|
|
Age:
|
embryonic day 11.0
|
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
2% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS18: brain blood vessel |
Present |
Regionally restricted |
2E
|
Authors report irregular network formation.
|
Specimen
2F:
(close )
|
Genetic Background:
|
involves: C57BL/6 * CHMU/Le
|
|
Mutant Allele(s):
|
Foxc1ch/Foxc1ch
|
|
Age:
|
embryonic day 11.0
|
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
2% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS19: brain blood vessel |
Present |
Regionally restricted |
2F
|
Formation of subventricular vascular plexus in deeper layer from the ventricular surface compared to wild type. Clearly delineated connections between vessels were not observed.
|
Specimen
2G:
embryonic day 11.5
(more )
Specimen
2G:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 11.5
|
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
2% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS19: brain blood vessel |
Present |
Regionally restricted |
2G
|
Authors report connected vascular networks.
|
Specimen
2H:
embryonic day 11.5
(more )
Specimen
2H:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 11.5
|
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
2% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS19: brain blood vessel |
Present |
Regionally restricted |
2H
|
Side view shows formation of subventricular vascular plexus.
|
Specimen
2I:
(close )
|
Genetic Background:
|
involves: C57BL/6 * CHMU/Le
|
|
Mutant Allele(s):
|
Foxc1ch/Foxc1ch
|
|
Age:
|
embryonic day 11.5
|
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
2% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS19: brain blood vessel |
Present |
Regionally restricted |
2I
|
Some regions in subventricular vascular plexus did not form regular networks. Authors report often clot-like staining was observed.
|
Specimen
2J:
(close )
|
Genetic Background:
|
involves: C57BL/6 * CHMU/Le
|
|
Mutant Allele(s):
|
Foxc1ch/Foxc1ch
|
|
Age:
|
embryonic day 11.5
|
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
2% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS19: brain blood vessel |
Present |
Regionally restricted |
2J
|
Lateral view show connections between vessels were not properly established.
|
|
Analysis Tools