Gene Expression Data
Assay Details
Assay
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Reference:
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J:207829
Dupuis LE, et al., Small leucine-rich proteoglycans exhibit unique spatiotemporal expression profiles during cardiac valve development. Dev Dyn. 2014 Apr;243(4):601-11
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6154373
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Gene symbol:
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Lum
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Gene name:
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lumican
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Results
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Specimen
1D:
embryonic day 10.5
(more )
Note: Double labeled: green - Lum; blue - Actc1 (assay MGI:6154957).
Specimen
1D:
(close )
Genetic Background:
|
involves: C57BL/6
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Age:
|
embryonic day 10.5
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Sex:
|
Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
|
Note:
|
Double labeled: green - Lum; blue - Actc1 (assay MGI:6154957).
|
Specimen
1D':
embryonic day 10.5
(more )
Note: Double labeled: green - Lum; blue - Actc1 (assay MGI:6154957).
Specimen
1D':
(close )
Genetic Background:
|
involves: C57BL/6
|
Age:
|
embryonic day 10.5
|
Sex:
|
Not Specified
|
Type:
|
section
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Fixation:
|
4% Paraformaldehyde
|
Note:
|
Double labeled: green - Lum; blue - Actc1 (assay MGI:6154957).
|
Specimen
1D'':
embryonic day 10.5
(more )
Note: Double labeled: green - Lum; blue - Actc1 (assay MGI:6154957).
Specimen
1D'':
(close )
Genetic Background:
|
involves: C57BL/6
|
Age:
|
embryonic day 10.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Double labeled: green - Lum; blue - Actc1 (assay MGI:6154957).
|
Specimen
2S:
embryonic day 14.5
(more )
Note: Double labeled: green - Lum; blue - Actc1 (assay MGI:6154957).
Specimen
2S:
(close )
Genetic Background:
|
involves: C57BL/6
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Double labeled: green - Lum; blue - Actc1 (assay MGI:6154957).
|
Specimen
2T:
embryonic day 14.5
(more )
Note: Double labeled: green - Lum; blue - Actc1 (assay MGI:6154957).
Specimen
2T:
(close )
Genetic Background:
|
involves: C57BL/6
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Double labeled: green - Lum; blue - Actc1 (assay MGI:6154957).
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: pulmonary artery |
Present |
Regionally restricted |
2T
|
Expression is in the adventitial layer.
|
Specimen
2U:
embryonic day 14.5
(more )
Note: Double labeled: green - Lum; blue - Actc1 (assay MGI:6154957).
Specimen
2U:
(close )
Genetic Background:
|
involves: C57BL/6
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Double labeled: green - Lum; blue - Actc1 (assay MGI:6154957).
|
Specimen
2V:
embryonic day 14.5
(more )
Note: Double labeled: green - Lum; blue - Actc1 (assay MGI:6154957).
Specimen
2V:
(close )
Genetic Background:
|
involves: C57BL/6
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Double labeled: green - Lum; blue - Actc1 (assay MGI:6154957).
|
Specimen
2W:
embryonic day 14.5
(more )
Note: Double labeled: green - Lum; blue - Actc1 (assay MGI:6154957). Amsterdam fixative.
Specimen
2W:
(close )
Genetic Background:
|
involves: C57BL/6
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Other - See Notes
|
Note:
|
Double labeled: green - Lum; blue - Actc1 (assay MGI:6154957). Amsterdam fixative.
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: great vessel of heart |
Present |
Regionally restricted |
2W
|
Expression is in the adventitial layer of the wall of the aorta. This is not seen in this specimen which has been fixed using Amsterdam fixative.
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Specimen
2X:
embryonic day 14.5
(more )
Note: Double labeled: green - Lum; blue - Actc1 (assay MGI:6154957). Amsterdam fixative.
Specimen
2X:
(close )
Genetic Background:
|
involves: C57BL/6
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Other - See Notes
|
Note:
|
Double labeled: green - Lum; blue - Actc1 (assay MGI:6154957). Amsterdam fixative.
|
Specimen
3P:
postnatal day 0
(more )
Note: Double labeled: green - Lum; blue - Actc1 (assay MGI:6154957). Amsterdam fixative.
Specimen
3P:
(close )
Genetic Background:
|
involves: C57BL/6
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Age:
|
postnatal day 0
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Sex:
|
Not Specified
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Type:
|
section
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Fixation:
|
Other - See Notes
|
Note:
|
Double labeled: green - Lum; blue - Actc1 (assay MGI:6154957). Amsterdam fixative.
|
Structure |
Level |
Pattern |
Image |
Note |
TS27: pulmonary artery |
Very strong |
Regionally restricted |
3P
,
3P'
|
Expression is at the site where the pulmonary valve cusps attach to the arterial wall.
|
TS27: pulmonary valve |
Very strong |
Regionally restricted |
3P
,
3P'
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Expression is at the site where the pulmonary valve cusps attach to the arterial wall.
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Specimen
3Q:
postnatal day 0
(more )
Note: Double labeled: green - Lum; blue - Actc1 (assay MGI:6154957).
Specimen
3Q:
(close )
Genetic Background:
|
involves: C57BL/6
|
Age:
|
postnatal day 0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Double labeled: green - Lum; blue - Actc1 (assay MGI:6154957).
|
Structure |
Level |
Pattern |
Image |
Note |
TS27: pulmonary artery |
Very strong |
Regionally restricted |
3Q
|
Expression is at the site where the pulmonary valve cusps attach to the arterial wall.
|
TS27: pulmonary valve |
Very strong |
Regionally restricted |
3Q
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Expression is at the site where the pulmonary valve cusps attach to the arterial wall.
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Specimen
3R:
postnatal day 0
(more )
Note: Double labeled: green - Lum; blue - Actc1 (assay MGI:6154957). Amsterdam fixative.
Specimen
3R:
(close )
Genetic Background:
|
involves: C57BL/6
|
Age:
|
postnatal day 0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Other - See Notes
|
Note:
|
Double labeled: green - Lum; blue - Actc1 (assay MGI:6154957). Amsterdam fixative.
|
Specimen
3S:
postnatal day 0
(more )
Note: Double labeled: green - Lum; blue - Actc1 (assay MGI:6154957). Amsterdam fixative.
Specimen
3S:
(close )
Genetic Background:
|
involves: C57BL/6
|
Age:
|
postnatal day 0
|
Sex:
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Not Specified
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Type:
|
section
|
Fixation:
|
Other - See Notes
|
Note:
|
Double labeled: green - Lum; blue - Actc1 (assay MGI:6154957). Amsterdam fixative.
|
Structure |
Level |
Pattern |
Image |
Note |
TS27: mitral valve |
Present |
Regionally restricted |
3S
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Expression is the leaflets but is absent in the region where the leaflets anchor into the myocardium.
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Specimen
3T:
postnatal day 0
(more )
Note: Double labeled: green - Lum; blue - Actc1 (assay MGI:6154957). Amsterdam fixative.
Specimen
3T:
(close )
Genetic Background:
|
involves: C57BL/6
|
Age:
|
postnatal day 0
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Sex:
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Not Specified
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Type:
|
section
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Fixation:
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Other - See Notes
|
Note:
|
Double labeled: green - Lum; blue - Actc1 (assay MGI:6154957). Amsterdam fixative.
|
Structure |
Level |
Pattern |
Image |
Note |
TS27: pericardium |
Present |
Regionally restricted |
3T
|
Expression is subjacent to the epicardial epithelial cell layer.
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Specimen
4M:
postnatal month 1
(more )
Note: Double labeled: green - Lum; blue - Actc1 (assay MGI:6154957). Amsterdam fixative.
Specimen
4M:
(close )
Genetic Background:
|
involves: C57BL/6
|
Age:
|
postnatal month 1
|
Sex:
|
Not Specified
|
Type:
|
section
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Fixation:
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Other - See Notes
|
Note:
|
Double labeled: green - Lum; blue - Actc1 (assay MGI:6154957). Amsterdam fixative.
|
Specimen
4N:
postnatal month 1
(more )
Note: Double labeled: green - Lum; blue - Actc1 (assay MGI:6154957). Amsterdam fixative.
Specimen
4N:
(close )
Genetic Background:
|
involves: C57BL/6
|
Age:
|
postnatal month 1
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Other - See Notes
|
Note:
|
Double labeled: green - Lum; blue - Actc1 (assay MGI:6154957). Amsterdam fixative.
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: aortic valve |
Present |
Regionally restricted |
4N
,
4N'
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Expression is in the distal cusps and is absent the valve hinge area (shown).
|
TS28: aorta tunica media |
Present |
Regionally restricted |
4N''
,
4N
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Expression is in the outer aspect.
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Specimen
4O:
postnatal month 1
(more )
Note: Double labeled: green - Lum; blue - Actc1 (assay MGI:6154957).
Specimen
4O:
(close )
Genetic Background:
|
involves: C57BL/6
|
Age:
|
postnatal month 1
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Double labeled: green - Lum; blue - Actc1 (assay MGI:6154957).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: aortic valve |
Present |
Regionally restricted |
4O
|
Expression is in the distal cusps.
|
Specimen
4P:
postnatal month 1
(more )
Note: Double labeled: green - Lum; blue - Actc1 (assay MGI:6154957). Amsterdam fixative.
Specimen
4P:
(close )
Genetic Background:
|
involves: C57BL/6
|
Age:
|
postnatal month 1
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Other - See Notes
|
Note:
|
Double labeled: green - Lum; blue - Actc1 (assay MGI:6154957). Amsterdam fixative.
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: mitral valve |
Present |
Regionally restricted |
4P
|
Expression is concentrated in the tip of the anterior leaflet is virtually absent in fibrous attachment of the posterior leaflet.
|
TS28: chordae tendinae |
Present |
|
4P
|
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Specimen
5J:
postnatal adult
(more )
Note: Double labeled: green - Lum; blue - Actc1 (assay MGI:6154957). Amsterdam fixative.
Specimen
5J:
(close )
Genetic Background:
|
involves: C57BL/6
|
Age:
|
postnatal adult
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Other - See Notes
|
Note:
|
Double labeled: green - Lum; blue - Actc1 (assay MGI:6154957). Amsterdam fixative.
|
Specimen
5K:
postnatal adult
(more )
Note: Double labeled: green - Lum; blue - Actc1 (assay MGI:6154957). Amsterdam fixative.
Specimen
5K:
(close )
Genetic Background:
|
involves: C57BL/6
|
Age:
|
postnatal adult
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Other - See Notes
|
Note:
|
Double labeled: green - Lum; blue - Actc1 (assay MGI:6154957). Amsterdam fixative.
|
Specimen
5L:
postnatal adult
(more )
Note: Double labeled: green - Lum; blue - Actc1 (assay MGI:6154957). Amsterdam fixative.
Specimen
5L:
(close )
Genetic Background:
|
involves: C57BL/6
|
Age:
|
postnatal adult
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Other - See Notes
|
Note:
|
Double labeled: green - Lum; blue - Actc1 (assay MGI:6154957). Amsterdam fixative.
|
|