Probe:	Bmp4 cDNA5
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS13: embryo mesoderm	Strong	Regionally restricted	1D	Expression is in the posterior mesoderm.
TS13: future prosencephalon	Strong	Regionally restricted	1D	Expression is in the prospective diencephalon.
TS13: amnion	Present		1D
TS13: future midbrain neural fold	Present		1D
TS13: future prosencephalon neural fold	Present		1D
TS13: surface ectoderm	Present		1D
TS13: yolk sac	Present	Regionally restricted	1D	Expression is in the mesodermal component.

Structure	Level	Pattern	Image	Note
TS13: trunk	Present	Regionally restricted	2D	Expression is in the body wall surrounding the pericardial cavity.

Structure	Level	Pattern	Image	Note
TS15: optic cup	Strong	Regionally restricted	3B	Expression is in the future neural retina.
TS15: optic vesicle	Strong	Regionally restricted	3B	Expression is in the anterior region.
TS15: neural crest	Strong	Regionally restricted	3B	Expression is in cephalic neural crest.
TS15: lens placode	Absent		3B

Structure	Level	Pattern	Image	Note
TS15: optic cup	Strong	Regionally restricted	3D	Expression is in the future neural retina.
TS15: optic vesicle	Strong	Regionally restricted	3D	Expression is in the anterior region.
TS15: neural crest	Strong	Regionally restricted	3D	Expression is in cephalic neural crest.
TS15: lens placode	Absent		3D

Structure	Level	Pattern	Image	Note
TS19: neural retina	Present	Regionally restricted	4B	Expression is in the anterior neural retina.
TS19: optic stalk	Present		4B
TS19: head mesenchyme	Present	Regionally restricted	4B	Expression is in mesenchyme surrounding the eye.

Structure	Level	Pattern	Image	Note
TS19: metanephros	Present	Regionally restricted	5C	Expression is surrounding the metanephric mesenchyme.
TS19: ureteric bud	Present		5C

Structure	Level	Pattern	Image	Note
TS21: comma-shaped body	Present		6D
TS21: mesenchyme of metanephros	Present		6D
TS21: s-shaped body	Present		6D
TS21: renal cortex	Present	Regionally restricted	6D	Expression is in the cell layer forming Bowman's capsule and in the future podocytes.

Structure	Level	Pattern	Image	Note
TS21: renal cortex	Present	Regionally restricted	7C	Expression is in Bowman's capsules and is absent in the podocytes.
TS21: early proximal tubule	Present		7C

Structure	Level	Pattern	Image	Note
TS21: ureteral mesenchyme	Present		7E

Structure	Level	Pattern	Image	Note
TS21: renal tubule	Present	Regionally restricted	9C	Expression is in the proximal part. Expression in this mutant is the same as in wild-type.
TS21: urinary system mesenchyme	Present	Regionally restricted	9C	Expression is in the mesenchyme surrounding the ureter. Expression in this mutant is the same as in wild-type.

Structure	Level	Pattern	Image	Note
TS19: metanephric mesenchyme	Absent			Absent in the metanephric mesenchyme and ureter.

Structure	Level	Pattern	Image	Note
TS21: metanephros	Present	Regionally restricted		Expression is along the ureter and collecting ducts extending into the nephrogenic zone.