Gene Expression Data
Assay Details
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Assay
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Reference:
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J:104848
Lin Y, et al., Dazl deficiency leads to embryonic arrest of germ cell development in XY C57BL/6 mice. Dev Biol. 2005 Dec 15;288(2):309-16
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6153933
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Gene symbol:
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Ddx4
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Gene name:
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DEAD box helicase 4
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Antibody:
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anti-MVH
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Detection system:
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Secondary antibody
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Results
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Specimen
2E:
postnatal day 0.5
(more  )
Specimen
2E:
(close  )
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Genetic Background:
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involves: C57BL/6
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Age:
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postnatal day 0.5
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
2F:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6
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Mutant Allele(s):
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Dazltm1Hjc/Dazltm1Hjc
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Age:
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postnatal day 0.5
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: germ cell of testis |
Present |
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2F
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| TS27: testis |
Present |
Single cells |
2F
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Authors report fewer than 10 expressing cells in 6 of 18 sections examined. (See Supplementary Fig S1)
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Specimen
not shown:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6
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Mutant Allele(s):
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Dazltm1Hjc/Dazltm1Hjc
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Age:
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postnatal day 0.5
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: testis |
Absent |
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Authors state there was no expression in 12 of 18 sections examined (see Supplementary Fig. S1.)
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Specimen
4G:
embryonic day 14.5
(more )
Specimen
4G:
(close )
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Genetic Background:
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involves: C57BL/6
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Age:
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embryonic day 14.5
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
4H:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6
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Mutant Allele(s):
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Dazltm1Hjc/Dazltm1Hjc
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Age:
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embryonic day 14.5
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: testis |
Trace |
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4H
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Expression greately reduced in mutant gonads compared to wildtype controls.
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Analysis Tools
