Probe:	Wif1 cDNA7
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS21: urogenital ridge	Trace		1B

Structure	Level	Pattern	Image	Note
TS21: urogenital ridge	Present	Regionally restricted	1C	Clear expression along entire length of Mullerian duct.
TS21: paramesonephric duct of male	Present	Widespread	1C	Clear expression along entire length of Mullerian duct.
TS21: testis	Present		1C

Structure	Level	Pattern	Image	Note
TS21: urogenital ridge	Present	Regionally restricted	1D	Clear expression along entire length of Mullerian duct.
TS21: paramesonephric duct of male	Present	Widespread	1D	Clear expression along entire length of Mullerian duct.
TS21: testis	Present		1D

Structure	Level	Pattern	Image	Note
TS21: mesenchyme of paramesonephric duct of male	Absent		1F
TS21: epithelium of paramesonephric duct of male	Trace		1F	Authors state that what little expression was observed localized to epithelial cells.

Structure	Level	Pattern	Image	Note
TS21: mesenchyme of paramesonephric duct of male	Present	Regionally restricted	1G	Expression localized to mesenchyme surrounding Mullerian duct epithelium.
TS21: epithelium of paramesonephric duct of male	Present		1G

Structure	Level	Pattern	Image	Note
TS21: mesenchyme of paramesonephric duct of male	Present	Regionally restricted	1H	Expression localized to mesenchyme surrounding Mullerian duct epithelium.
TS21: epithelium of paramesonephric duct of male	Present		1H

Structure	Level	Pattern	Image	Note
TS20: urogenital ridge	Absent		2A

Structure	Level	Pattern	Image	Note
TS21: mesenchyme of paramesonephric duct of male	Strong	Regionally restricted	2B	Strong expression in mesenchyme surrounding Mullerian duct epithelium, particularly at cranial end.

Structure	Level	Pattern	Image	Note
TS22: paramesonephric duct of male	Present	Regionally restricted	2C	Expression in middle to caudal regions where Mullerian duct regression has not occured
TS22: degenerating rest of paramesonephric duct of male	Absent		2C	No expression in rostral (cranial) sections where Mullerian duct has regressed.

Structure	Level	Pattern	Image	Note
TS20: paramesonephric duct of male	Absent		2D

Structure	Level	Pattern	Image	Note
TS21: mesenchyme of paramesonephric duct of male	Strong	Regionally restricted	2E	Strong expression in mesenchyme surrounding Mullerian duct epithelium, particularly at cranial end.

Structure	Level	Pattern	Image	Note
TS22: degenerating rest of paramesonephric duct of male	Absent		2F	No expression in rostral (cranial) sections where Mullerian duct has regressed.

Structure	Level	Pattern	Image	Note
TS22: paramesonephric duct of male	Present	Regionally restricted	2G	Expression in caudal regions where Mullerian duct has not regressed.

Structure	Level	Pattern	Image	Note
TS21: urogenital ridge	Absent		2H

Structure	Level	Pattern	Image	Note
TS21: paramesonephric duct of female	Absent		2I