Gene Expression Data
Assay Details
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Assay
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Reference:
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J:253968
Abdelhamed Z, et al., A mutation in Ccdc39 causes neonatal hydrocephalus with abnormal motile cilia development in mice. Development. 2018 Jan 9;145(1):dev154500
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6147588
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Gene symbol:
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Gas8
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Gene name:
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growth arrest specific 8
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Antibody:
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Gas8 antibody
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Detection system:
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Secondary antibody coupled to Alexa Fluor 488
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Results
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Specimen
6B WT GAS8:
postnatal day 1
(more  )
Note: Triple labeled: green - Gas8; red - acetylated tubulin; blue - DAPI.
Specimen
6B WT GAS8:
(close  )
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Genetic Background:
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Not Specified
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Age:
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postnatal day 1
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Triple labeled: green - Gas8; red - acetylated tubulin; blue - DAPI.
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Specimen
6B prh/prh GAS8:
postnatal day 1; Ccdc39prh/ Ccdc39prh
(more )
Note: Triple labeled: green - Gas8; red - acetylated tubulin; blue - DAPI.
Specimen
6B prh/prh GAS8:
(close )
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Genetic Background:
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involves: A/J * FVB/N
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Mutant Allele(s):
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Ccdc39prh/Ccdc39prh
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Age:
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postnatal day 1
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Triple labeled: green - Gas8; red - acetylated tubulin; blue - DAPI.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: ependyma |
Present |
Regionally restricted |
6B prh/prh merged
,
6B prh/prh GAS8
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Expression only rarely detected near apical cytoplasm of mutant ependymal cells. Expression never localized to mutant axoneme.
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Specimen
S6C WT GAS8:
postnatal day 1
(more )
Note: Triple labeled: green - Gas8; red - acetylated tubulin; blue - DAPI.
Specimen
S6C WT GAS8:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal day 1
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Triple labeled: green - Gas8; red - acetylated tubulin; blue - DAPI.
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Specimen
S6C prh/prh GAS8:
postnatal day 1; Ccdc39prh/ Ccdc39prh
(more )
Note: Triple labeled: green - Gas8; red - acetylated tubulin; blue - DAPI.
Specimen
S6C prh/prh GAS8:
(close )
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Genetic Background:
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involves: A/J * FVB/N
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Mutant Allele(s):
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Ccdc39prh/Ccdc39prh
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Age:
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postnatal day 1
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Triple labeled: green - Gas8; red - acetylated tubulin; blue - DAPI.
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Specimen
S7 WT GAS8:
postnatal day 3
(more )
Note: Triple labeled: green - Gas8; red - acetylated tubulin; blue - DAPI.
Specimen
S7 WT GAS8:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal day 3
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Triple labeled: green - Gas8; red - acetylated tubulin; blue - DAPI.
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Specimen
S7 prh/prh GAS8:
postnatal day 3; Ccdc39prh/ Ccdc39prh
(more )
Note: Triple labeled: green - Gas8; red - acetylated tubulin; blue - DAPI.
Specimen
S7 prh/prh GAS8:
(close )
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Genetic Background:
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involves: A/J * FVB/N
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Mutant Allele(s):
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Ccdc39prh/Ccdc39prh
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Age:
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postnatal day 3
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Triple labeled: green - Gas8; red - acetylated tubulin; blue - DAPI.
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Analysis Tools