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Gene Expression Data
Assay Details
Assay
Reference: J:253968 Abdelhamed Z, et al., A mutation in Ccdc39 causes neonatal hydrocephalus with abnormal motile cilia development in mice. Development. 2018 Jan 9;145(1):dev154500
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6147587
Gene symbol: Dnali1
Gene name: dynein, axonemal, light intermediate polypeptide 1
Antibody: Dnali1 antibody
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen S4 E16.5 DNALI1: embryonic day 16.5 (more )
Note: Triple labeled: green - Dnali1; red - acetylated tubulin; blue - DAPI.
Structure Level Pattern Image Note
TS24: lateral ventricle Present Regionally restricted S4_E16.5 , S4_E16.5 merged , S4_E16.5 DNALlI1 Cytoplasmic and nascent ciliary expression in fractions of ependymal cells on medial side of forebrain ventricles.
Specimen S4 P0 DNALI1: postnatal day 0 (more )
Note: Triple labeled: green - Dnali1; red - acetylated tubulin; blue - DAPI.
Structure Level Pattern Image Note
TS27: ependyma Present Widespread S4_P0 merged , S4_P0 , S4_P0 DNALI1 More robust expression in most ependymal cells in both cytoplasm and cilia.
Specimen 6A WT DNALI1: postnatal day 1 (more )
Note: Triple labeled: green - Dnali1; red - acetylated tubulin; blue - DAPI.
Structure Level Pattern Image Note
TS27: ependyma Present Regionally restricted 6A WT (boxed area) , 6A WT DNALI1 , 6A WT merged , 6A WT Authors report co-expression with acetylated alpha-tubulin within axoneme of wild-type ependymal cells on medial ventricular wall.
Specimen 6A prh/prh DNALI1: postnatal day 1; Ccdc39prh/Ccdc39prh (more )
Note: Triple labeled: green - Dnali1; red - acetylated tubulin; blue - DAPI.
Structure Level Pattern Image Note
TS27: ependyma Present Regionally restricted 6A prh/prh merged , 6A prh/prh , 6A prh/prh (boxed area) , 6A prh/prh DNALI1 No co-expression with acetylated alpha-tubulin within axoneme of mutant ependymal cells, while cytoplasmic expression appeared normal or slightly enhanced.
Specimen S5 WT DNALI1: postnatal day 9 (more )
Note: Triple labeled: green - Dnali1; red - acetylated tubulin; blue - DAPI.
Structure Level Pattern Image Note
TS28: ependyma Present Regionally restricted S5_WT Merged , S5_WT DNALI1 Expression in ependymal cells in forebrain.
Specimen S5 prh/prh DNALI1: postnatal day 9; Ccdc39prh/Ccdc39prh (more )
Note: Triple labeled: green - Dnali1; red - acetylated tubulin; blue - DAPI.
Structure Level Pattern Image Note
TS28: ependyma Present Regionally restricted S5_prh/prh Merged , S5_prh/prh DNALI1 Expression in ependymal cells in forebrain. No expression in axoneme of mutant ependymal cilia. Abnormal cytoplasmic accumulation of expression in mutant cells.
Specimen S6A WT DNALI1: postnatal day 1 (more )
Note: Triple labeled: green - Dnali1; red - acetylated tubulin; blue - DAPI.
Structure Level Pattern Image Note
TS27: choroid plexus Present S6A WT merged , S6A WT , S6A WT DNALI1
Specimen S6A prh/prh DNALI1: postnatal day 1; Ccdc39prh/Ccdc39prh (more )
Note: Triple labeled: green - Dnali1; red - acetylated tubulin; blue - DAPI.
Structure Level Pattern Image Note
TS27: choroid plexus Present S6A prh/prh merged , S6A prh/prh DNAL1A , S6A prh/prh
Specimen S6B WT DNALI1: postnatal day 1 (more )
Note: Triple labeled: green - Dnali1; red - acetylated tubulin; blue - DAPI.
Structure Level Pattern Image Note
TS27: choroid plexus Present Regionally restricted S6B WT Expression localized at base of cilia in both wild-type and mutant cilia.
Specimen S6B prh/prh DNALI1: postnatal day 1; Ccdc39prh/Ccdc39prh (more )
Note: Triple labeled: green - Dnali1; red - acetylated tubulin; blue - DAPI.
Structure Level Pattern Image Note
TS27: choroid plexus Present Regionally restricted S6B prh/prh Expression localized at base of cilia in both wild-type and mutant cilia.
Specimen S7 WT DNALI1: postnatal day 3 (more )
Note: Triple labeled: green - Dnali1; red - acetylated tubulin; blue - DAPI.
Structure Level Pattern Image Note
TS27: bronchus Present Regionally restricted S7_WT (top) , S7_WT DNAL1 , S7_WT merged (top) Expression in axoneme of bronchial cilia.
Specimen S7 prh/prh DNALI1: postnatal day 3; Ccdc39prh/Ccdc39prh (more )
Note: Triple labeled: green - Dnali1; red - acetylated tubulin; blue - DAPI.
Structure Level Pattern Image Note
TS27: bronchus Present Regionally restricted S7_prh/prh DNALI1 , S7_prh/prh merged (top) , S7_prh/prh (top) No expression in axoneme of mutant bronchial cilia.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
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