Gene Expression Data
Assay Details
Assay
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Reference:
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J:227089
Pirson M, et al., Expression of peroxiredoxins and thioredoxins in the mouse spinal cord during embryonic development. J Comp Neurol. 2015 Dec 1;523(17):2599-617
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6144136
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Gene symbol:
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Prdx2
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Gene name:
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peroxiredoxin 2
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Antibody:
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Prdx2 antibody
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Detection system:
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Secondary antibody coupled to Alexa Fluor 488
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Results
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Specimen
1B:
embryonic day 16.5
(more )
Specimen
1B:
(close )
Genetic Background:
|
C57BL/6N
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Age:
|
embryonic day 16.5
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Sex:
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Not Specified
|
Type:
|
section
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Fixation:
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4% Paraformaldehyde
|
Embedding:
|
Cryosection
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Specimen
4A:
embryonic day 10.5
(more )
Specimen
4A:
(close )
Genetic Background:
|
C57BL/6N
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Age:
|
embryonic day 10.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: future spinal cord |
Strong |
Regionally restricted |
4A
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Cytosolic expression, more intense in ventral and lateral areas of spinal cord. Strong expression in single cell located throughout spinal cord.
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Specimen
4B:
embryonic day 11.5
(more )
Specimen
4B:
(close )
Genetic Background:
|
C57BL/6N
|
Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: future spinal cord |
Strong |
Regionally restricted |
4B
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Nuclear expression in ventral part of spinal cord, above cytosolic expression seen at previous stage.
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Specimen
4C:
embryonic day 12.5
(more )
Specimen
4C:
(close )
Genetic Background:
|
C57BL/6N
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: spinal cord |
Strong |
Regionally restricted |
4C
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Nuclear expression in ventral part of spinal cord, above cytosolic expression seen at previous stage.
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Specimen
4D:
embryonic day 13.5
(more )
Specimen
4D:
(close )
Genetic Background:
|
C57BL/6N
|
Age:
|
embryonic day 13.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: spinal cord |
Present |
Widespread |
4D
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Nuclear expression extending to entire spinal cord.
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TS21: dorsal root ganglion |
Strong |
Regionally restricted |
4D
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Strong expression in nuclei of dorsal root ganglion neurons.
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Specimen
4E:
embryonic day 14.5
(more )
Specimen
4E:
(close )
Genetic Background:
|
C57BL/6N
|
Age:
|
embryonic day 14.5
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Sex:
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Not Specified
|
Type:
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section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: spinal cord |
Present |
Widespread |
4E
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Nuclear expression extending to entire spinal cord.
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Specimen
4F:
embryonic day 16.5
(more )
Specimen
4F:
(close )
Genetic Background:
|
C57BL/6N
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Age:
|
embryonic day 16.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS24: spinal cord |
Strong |
Regionally restricted |
4F
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Intense nuclear expression in ventrally-located cell population.
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Specimen
4G:
embryonic day 18.5
(more )
Specimen
4G:
(close )
Genetic Background:
|
C57BL/6N
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Age:
|
embryonic day 18.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS26: spinal cord |
Strong |
Regionally restricted |
4G
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Intense nuclear expression in ventrally-located cell population.
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Specimen
4H/K:
embryonic day 12.5
(more )
Note: Triple labeled: green - Prdx2; purple - Foxp1 (assay MGI:6144147); red - Isl1 (assay MGI:6144145) and Isl2 (assay MGI:6144251).
Specimen
4H/K:
(close )
Genetic Background:
|
C57BL/6N
|
Age:
|
embryonic day 12.5
|
Sex:
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Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Triple labeled: green - Prdx2; purple - Foxp1 (assay MGI:6144147); red - Isl1 (assay MGI:6144145) and Isl2 (assay MGI:6144251).
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: spinal cord |
Strong |
Regionally restricted |
4H
,
4K
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Authors report strong ventral expression co-localized in nucleus with motor neuron markers Isl1/2 and Foxp1.
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Specimen
4L/O:
embryonic day 12.5
(more )
Note: Triple labeled: green - Prdx2; purple - Foxp1 (assay MGI:6144147); red - Isl1 (assay MGI:6144145) and Isl2 (assay MGI:6144251).
Specimen
4L/O:
(close )
Genetic Background:
|
C57BL/6N
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Age:
|
embryonic day 12.5
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Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Triple labeled: green - Prdx2; purple - Foxp1 (assay MGI:6144147); red - Isl1 (assay MGI:6144145) and Isl2 (assay MGI:6144251).
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: spinal cord |
Strong |
Regionally restricted |
4O
,
4L
|
Authors report strong ventral expression co-localized in nucleus with motor neuron markers Isl1/2 and Foxp1.
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Specimen
4P/S:
embryonic day 16.5
(more )
Note: Triple labeled: green - Prdx2; purple - Foxp1 (assay MGI:6144147); red - Isl1 (assay MGI:6144145) and Isl2 (assay MGI:6144251).
Specimen
4P/S:
(close )
Genetic Background:
|
C57BL/6N
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Age:
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embryonic day 16.5
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Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Triple labeled: green - Prdx2; purple - Foxp1 (assay MGI:6144147); red - Isl1 (assay MGI:6144145) and Isl2 (assay MGI:6144251).
|
Structure |
Level |
Pattern |
Image |
Note |
TS24: spinal cord |
Strong |
Regionally restricted |
4S
,
4P
|
Authors report strong ventral expression co-localized in nucleus with motor neuron markers Isl1/2 and Foxp1.
|
Specimen
4T/W:
embryonic day 16.5
(more )
Note: Triple labeled: green - Prdx2; purple - Foxp1 (assay MGI:6144147); red - Isl1 (assay MGI:6144145) and Isl2 (assay MGI:6144251).
Specimen
4T/W:
(close )
Genetic Background:
|
C57BL/6N
|
Age:
|
embryonic day 16.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Triple labeled: green - Prdx2; purple - Foxp1 (assay MGI:6144147); red - Isl1 (assay MGI:6144145) and Isl2 (assay MGI:6144251).
|
Structure |
Level |
Pattern |
Image |
Note |
TS24: spinal cord |
Strong |
Regionally restricted |
4T
,
4W
|
Authors report strong ventral expression co-localized in nucleus with motor neuron markers Isl1/2 and Foxp1.
|
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