Gene Expression Data
Assay Details
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Assay
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Reference:
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J:19896
Weinstein DC, et al., The winged-helix transcription factor HNF-3 beta is required for notochord development in the mouse embryo. Cell. 1994 Aug 26;78(4):575-88
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6144039
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Gene symbol:
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Lhx5
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Gene name:
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LIM homeobox protein 5
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Antibody:
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4F2
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Detection system:
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Secondary antibody coupled to horseradish peroxidase
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Assay notes:
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The specificity of the antibody used was not detailed; both family members have been annotated.
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Results
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Specimen
6E:
embryonic day 9.5; Foxa2tm1Dnl/Foxa2 +
(more  )
Specimen
6E:
(close  )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6J
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Mutant Allele(s):
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Foxa2tm1Dnl/Foxa2+
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: neural tube lateral wall |
Present |
Regionally restricted |
6E
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Expressed in the nuclei of cells in the intermediate zone of the neural tube and close to the extreme limiting membrane.
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Specimen
6F:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6J
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Mutant Allele(s):
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Foxa2tm1Dnl/Foxa2tm1Dnl
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: neural tube lateral wall |
Present |
Regionally restricted |
6F
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Expressed in a position equivalent to that in heterozygotes.
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Specimen
6G:
embryonic day 9.5; Foxa2tm1Dnl/Foxa2 +
(more )
Specimen
6G:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6J
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Mutant Allele(s):
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Foxa2tm1Dnl/Foxa2+
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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Specimen
6H:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6J
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Mutant Allele(s):
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Foxa2tm1Dnl/Foxa2tm1Dnl
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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Analysis Tools
