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Gene Expression Data
Assay Details
Assay
Reference: J:19896 Weinstein DC, et al., The winged-helix transcription factor HNF-3 beta is required for notochord development in the mouse embryo. Cell. 1994 Aug 26;78(4):575-88
Assay type: In situ reporter (knock in)
MGI Accession ID: MGI:6143844
Gene symbol: Foxa2
Gene name: forkhead box A2
Reporter: lacZ
Direct Detection
Results
Specimen 1D: embryonic day 7.5; Foxa2tm1Dnl/Foxa2+ (more )
Note: Fixed in 1% formaldehyde, 0.2% glutaraldehyde.
Structure Level Pattern Image Note
TS11: embryo endoderm Strong 1D
TS11: node Strong 1D
TS11: notochordal process Strong 1D
TS11: extraembryonic endoderm Trace 1D Very low levels.
TS11: prechordal plate Present 1D
Specimen 1E: embryonic day 9.5; Foxa2tm1Dnl/Foxa2+ (more )
Note: Fixed in 1% formaldehyde, 0.2% glutaraldehyde.
Structure Level Pattern Image Note
TS15: gut Present 1E
TS15: hepatic primordium Present 1E
TS15: neural tube floor plate Present 1E
TS15: notochord Present 1E
TS15: pharynx Present 1E
TS15: axial mesoderm Present Regionally restricted 1E Expressed in the node.
Specimen 2A: embryonic day 6.5; Foxa2tm1Dnl/Foxa2+ (more )
Note: Fixed in 1% formaldehyde, 0.2% glutaraldehyde.
Structure Level Pattern Image Note
TS9: primitive streak Present Regionally restricted 2A Expressed in cells at the tip of the primitive streak, the precursors of the node.
Specimen 2B: embryonic day 6.5; Foxa2tm1Dnl/Foxa2+ (more )
Note: Fixed in 1% formaldehyde, 0.2% glutaraldehyde.
Structure Level Pattern Image Note
TS9: primitive streak Weak Scattered 2B
Specimen 2C: embryonic day 7.5; Foxa2tm1Dnl/Foxa2+ (more )
Note: Fixed in 1% formaldehyde, 0.2% glutaraldehyde.
Structure Level Pattern Image Note
TS11: node Strong 2C
TS11: notochordal process Strong 2C
TS11: neural plate Strong Regionally restricted 2C Expressed in the midline cells.
TS11: embryo endoderm Present Regionally restricted 2C Expressed in the endodermal cells emigrating from the node.
TS11: prechordal plate Present 2C
Specimen 2D: embryonic day 8.5; Foxa2tm1Dnl/Foxa2+ (more )
Note: Fixed in 1% formaldehyde, 0.2% glutaraldehyde.
Structure Level Pattern Image Note
TS12: neural tube floor plate Present Regionally restricted 2D Expressed in the ventral midline.
TS12: foregut diverticulum endoderm Present 2D
TS12: notochord Present 2D
TS12: node Strong 2D
Specimen 2E: embryonic day 9.5; Foxa2tm1Dnl/Foxa2+ (more )
Note: Fixed in 1% formaldehyde, 0.2% glutaraldehyde.
Structure Level Pattern Image Note
TS15: floor plate Present Patchy 2E With an anterior boundary in the posterior diencephalon.
TS15: notochord Present 2E
Specimen 2I: embryonic day 7.5; Foxa2tm1Dnl/Foxa2tm1Dnl (more )
Note: Fixed in 1% formaldehyde, 0.2% glutaraldehyde. Sample was after removal of the extraembryonic tissues and the parietal endoderm.
Structure Level Pattern Image Note
TS11: primitive streak Weak Scattered 2I Expressing cells were found scattered throughout the region.
Specimen 2K: embryonic day 9.5; Foxa2tm1Dnl/Foxa2+ (more )
Note: Fixed in 1% formaldehyde, 0.2% glutaraldehyde.
Structure Level Pattern Image Note
TS15: floor plate Present Regionally restricted 2K Expressed in the anterior.
Specimen 4F: embryonic day 9.5; Foxa2tm1Dnl/Foxa2tm1Dnl (more )
Note: Double labeled: blue - Foxa2 reporter; yellow - Foxa1 (assay MGI:6143863).
Structure Level Pattern Image Note
TS15: embryo Weak Scattered 4F
Specimen not shown: embryonic day 6.0; Foxa2tm1Dnl/Foxa2+ (more )
Note: Fixed in 1% formaldehyde, 0.2% glutaraldehyde.
Structure Level Pattern Image Note
TS8: epiblast Present Regionally restricted Expressed at the tip of the primitive streak, the future node.
Specimen not shown: embryonic day 7.0; Foxa2tm1Dnl/Foxa2+ (more )
Note: Fixed in 1% formaldehyde, 0.2% glutaraldehyde.
Structure Level Pattern Image Note
TS10: node Strong
TS10: embryo mesoderm Strong Regionally restricted Expressed in the node.
TS10: neural ectoderm Strong Regionally restricted Expressed in the midline neural plate.
Specimen not shown: embryonic day 9.5; Foxa2tm1Dnl/Foxa2tm1Dnl (more )
Structure Level Pattern Image Note
TS15: embryo endoderm Weak Scattered
TS15: embryo mesoderm Weak Scattered
TS15: neural ectoderm Weak Scattered
Specimen not shown: embryonic day 10.5; Foxa2tm1Dnl/Foxa2+ (more )
Note: Fixed in 1% formaldehyde, 0.2% glutaraldehyde.
Structure Level Pattern Image Note
TS17: gut Present
TS17: neural tube floor plate Present
TS17: notochord Present
TS17: hindbrain Weak Regionally restricted Expressed in the dorsal region, which is different from the endogenous expression.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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