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Gene Expression Data
Assay Details
Assay
Reference: J:244447 Li B, et al., Mid1/Mid2 expression in craniofacial development and a literature review of X-linked opitz syndrome. Mol Genet Genomic Med. 2016 Jan;4(1):95-105
Assay type: RNA in situ
MGI Accession ID: MGI:6121142
Gene symbol: Mid2
Gene name: midline 2
Probe: Mid2 oligo1
Probe preparation: Antisense labelled with digoxigenin RNA
Visualized with: Alkaline phosphatase
Results
Specimen 3: embryonic day 10.5 (more )
Structure Level Pattern Image Note
TS17: embryo Present Ubiquitous 3A
TS17: branchial arch Strong 3A
TS17: branchial arch ectoderm Strong 3A
TS17: forebrain Strong 3A
TS17: frontal process ectoderm Strong 3A
TS17: head surface ectoderm Strong 3A
TS17: heart Strong 3A
TS17: somite Strong 3A
TS17: vitelline duct Strong 3A
TS17: head Strong Regionally restricted 3A Expression is in the epithelium of the craniofacial prominences.
TS17: hindbrain Strong Regionally restricted 3A Expression is high in the anterior portion.

Specimen 4C: embryonic day 10.5 (more )
Structure Level Pattern Image Note
TS17: 1st branchial arch mandibular component ectoderm Strong 4C
TS17: 1st branchial arch maxillary component ectoderm Strong 4C
TS17: 1st branchial arch mandibular component mesenchyme Strong Regionally restricted 4C Expression is in the mesenchyme adjacent to the epithelium.
TS17: 1st branchial arch maxillary component mesenchyme Strong Regionally restricted 4C Expression is in the mesenchyme adjacent to the epithelium.
TS17: nasal pit epithelium Strong Regionally restricted 4C Expression is in the dorsal-most border of nasal pit.

Specimen 4D: embryonic day 10.5 (more )
Structure Level Pattern Image Note
TS17: latero-nasal process ectoderm Strong 4D Expression is stronger in the distal ends.
TS17: medial-nasal process ectoderm Strong 4D Expression is stronger in the distal ends.
TS17: latero-nasal process mesenchyme Strong Regionally restricted 4D Expression is in the mesenchyme adjacent to the epithelium and is stronger at the distal ends.
TS17: medial-nasal process mesenchyme Strong Regionally restricted 4D Expression is in the mesenchyme adjacent to the epithelium and is stronger at the distal ends.

Specimen 5E: embryonic day 10.5 (more )
Structure Level Pattern Image Note
TS17: latero-nasal process ectoderm Strong 5E Expression is stronger in the distal ends.
TS17: medial-nasal process ectoderm Strong 5E Expression is stronger in the distal ends.
TS17: latero-nasal process mesenchyme Strong Regionally restricted 5E Expression is in the mesenchyme adjacent to the epithelium. Expression is stronger in the distal ends.
TS17: medial-nasal process mesenchyme Strong Regionally restricted 5E Expression is in the mesenchyme adjacent to the epithelium. Expression is stronger in the distal ends.
TS17: forebrain Strong 5E
TS17: hindbrain Strong 5E

Specimen 5F: embryonic day 10.5 (more )
Structure Level Pattern Image Note
TS17: latero-nasal process ectoderm Strong Regionally restricted 5F Expression is stronger in the distal ends at the putative site of fusion.
TS17: latero-nasal process mesenchyme Strong Regionally restricted 5F Expression is stronger in the distal ends at the putative site of fusion.
TS17: medial-nasal process ectoderm Strong Regionally restricted 5F Expression is stronger in the distal ends at the putative site of fusion.
TS17: medial-nasal process mesenchyme Strong Regionally restricted 5F Expression is stronger in the distal ends at the putative site of fusion.

Specimen 5G: embryonic day 10.5 (more )
Structure Level Pattern Image Note
TS17: latero-nasal process ectoderm Weak Regionally restricted 5G After fusion expression at the distal ends is downregulated.
TS17: latero-nasal process mesenchyme Weak Regionally restricted 5G After fusion expression at the distal ends is downregulated.
TS17: medial-nasal process ectoderm Weak Regionally restricted 5G After fusion expression at the distal ends is downregulated.
TS17: medial-nasal process mesenchyme Weak Regionally restricted 5G After fusion expression at the distal ends is downregulated.


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
03/18/2025
MGI 6.24
The Jackson Laboratory