Fgf8 RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:88784 Petryk A, et al., The mammalian twisted gastrulation gene functions in foregut and craniofacial development. Dev Biol. 2004 Mar 15;267(2):374-86
Assay type:	RNA in situ
MGI Accession ID:	MGI:6118928
Gene symbol:	Fgf8
Gene name:	fibroblast growth factor 8

Probe:	Fgf8 cDNA13
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Results

5E ©

Specimen 5E: embryonic day 9.5 (more

)

Structure	Level	Pattern	Image	Note
TS15: telencephalon	Present	Regionally restricted	5E	Expression in commissural plate.
TS15: 1st branchial arch	Present		5E
TS15: midbrain-hindbrain junction	Present	Regionally restricted	5E	Expression in isthmic organizer.

5F ©

Specimen 5F: embryonic day 9.5; Twsg1^tm1.1Mboc/Twsg1^tm1.1Mboc (more

)
Note: Mildly affected mutant embryo.

Structure	Level	Pattern	Image	Note
TS15: telencephalon	Weak	Regionally restricted	5F	Reduced expression in commissural plate.
TS15: 1st branchial arch	Present		5F
TS15: midbrain-hindbrain junction	Present	Regionally restricted	5F	Normal expression in isthmic organizer.

5G ©

Specimen 5G: embryonic day 9.5 (more

)

Structure	Level	Pattern	Image	Note
TS15: telencephalon	Present	Regionally restricted	5G	Expression in commissural plate.
TS15: 1st branchial arch	Present		5G
TS15: midbrain-hindbrain junction	Present	Regionally restricted	5G	Expression in isthmic organizer.

5H ©

Specimen 5H: embryonic day 9.5 (more

)
Note: Severely affected mutant embryo. Rostral structures are absent.

Structure	Level	Pattern	Image	Note
TS15: midbrain-hindbrain junction	Present	Regionally restricted	5H	Normal expression in isthmic organizer.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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