Gene Expression Data
Assay Details
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Assay
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Reference:
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J:106304
Jeays-Ward K, et al., Wnt4 is required for proper male as well as female sexual development. Dev Biol. 2004 Dec 15;276(2):431-40
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:6117283
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Gene symbol:
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Sox9
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Gene name:
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SRY (sex determining region Y)-box 9
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Probe:
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Sox9 probe34
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Assay notes:
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Authors refer to specimens that are not homozygous Wnt4-/- as wild-type, but report they can be either +/+ or +/-, and report no differences were found between +/+ and Wnt+/- embryos for all genes analyzed at all stages.
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Results
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Specimen
1A Sox9 -/-:
(close  )
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Genetic Background:
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involves: 129S1/Sv * 129S7/SvEvBrd * C57BL/6 * CBA
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Mutant Allele(s):
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Wnt4tm1Amc/Wnt4tm1Amc
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Age:
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embryonic day 11.5
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Sex:
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Male
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: gonad |
Weak |
Regionally restricted |
1A Sox9 -/-
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Authors report a significant reduction in Sertoli cells in mutant compared with wild-type gonads.
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Specimen
1A Sox9 (right pane):
embryonic day 11.5
(more  )
Specimen
1A Sox9 (right pane):
(close )
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Genetic Background:
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involves: 129S1/Sv * 129S7/SvEvBrd * C57BL/6 * CBA
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Age:
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embryonic day 11.5
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Sex:
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Male
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
4B -/- (center pane):
(close )
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Genetic Background:
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involves: 129S1/Sv * 129S7/SvEvBrd * C57BL/6 * CBA
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Mutant Allele(s):
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Wnt4tm1Amc/Wnt4tm1Amc
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Age:
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embryonic day 11.5
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Sex:
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Male
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
4B +/-:
embryonic day 11.5; Wnt4tm1Amc/Wnt4 +
(more )
Specimen
4B +/-:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129S7/SvEvBrd * C57BL/6 * CBA
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Mutant Allele(s):
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Wnt4tm1Amc/Wnt4+
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Age:
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embryonic day 11.5
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Sex:
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Male
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
5c:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129S7/SvEvBrd * C57BL/6 * CBA
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Mutant Allele(s):
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Wnt4tm1Amc/Wnt4tm1Amc
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Age:
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embryonic day 14.5
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Sex:
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Male
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: testis |
Weak |
Regionally restricted |
5c
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Authors state there were fewer and more disorganized testicular cords in mutant embryos.
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Specimen
5d:
embryonic day 14.5
(more )
Specimen
5d:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129S7/SvEvBrd * C57BL/6 * CBA
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Age:
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embryonic day 14.5
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Sex:
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Male
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: testis |
Present |
Regionally restricted |
5d
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Specimen
6 Sox9 -/-:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129S7/SvEvBrd * C57BL/6 * CBA
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Mutant Allele(s):
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Wnt4tm1Amc/Wnt4tm1Amc
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Age:
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embryonic day 12.5
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Sex:
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Male
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
6 Sox9 (right pane):
embryonic day 12.5
(more )
Specimen
6 Sox9 (right pane):
(close )
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Genetic Background:
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involves: 129S1/Sv * 129S7/SvEvBrd * C57BL/6 * CBA
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Age:
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embryonic day 12.5
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Sex:
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Male
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Analysis Tools