Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:106304
Jeays-Ward K, et al., Wnt4 is required for proper male as well as female sexual development. Dev Biol. 2004 Dec 15;276(2):431-40
|
|
Assay type:
|
Immunohistochemistry
|
|
MGI Accession ID:
|
MGI:6117280
|
|
Gene symbol:
|
Pecam1
|
|
Gene name:
|
platelet/endothelial cell adhesion molecule 1
|
|
Antibody:
|
Anti-Pecam
|
|
Detection system:
|
horseradish peroxidase
|
|
Assay notes:
|
Authors refer to specimens that are not homozygous Wnt4-/- as wild-type, but report they can be either +/+ or +/-, and report no differences were found between +/+ and Wnt+/- embryos for all genes analyzed at all stages.
|
|
|
Results
|
Specimen
6 PECAM -/-:
(close  )
|
Genetic Background:
|
involves: 129S1/Sv * 129S7/SvEvBrd * C57BL/6 * CBA
|
|
Mutant Allele(s):
|
Wnt4tm1Amc/Wnt4tm1Amc
|
|
Age:
|
embryonic day 12.5
|
|
Sex:
|
Male
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS20: coelomic vessel |
Present |
Regionally restricted |
6_PECAM -/-
|
Authors state that the coelomic blood vessel coalesced into a single vessel earlier in the mutant gonad compared to wild-type.
|
Specimen
6 PECAM (right pane):
embryonic day 12.5
(more  )
Specimen
6 PECAM (right pane):
(close )
|
Genetic Background:
|
involves: 129S1/Sv * 129S7/SvEvBrd * C57BL/6 * CBA
|
|
Age:
|
embryonic day 12.5
|
|
Sex:
|
Male
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Analysis Tools
