Gene Expression Data
Assay Details
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Assay
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Reference:
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J:20949
Smith TH, et al., Somite subdomains, muscle cell origins, and the four muscle regulatory factor proteins. J Cell Biol. 1994 Oct;127(1):95-105
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6117038
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Gene symbol:
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Myf6
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Gene name:
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myogenic factor 6
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Antibody:
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MRF4 antibody
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
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Results
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Specimen
3E:
embryonic day 9.5
(more  )
Note: 24-26 somite stage embryo.
Specimen
3E:
(close  )
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Genetic Background:
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CD-1
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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24-26 somite stage embryo.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: somite 10 |
Present |
Regionally restricted |
3E
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Expression is mostly ventral.
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| TS15: somite 11 |
Present |
Regionally restricted |
3E
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Expression is mostly ventral.
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Specimen
6C:
embryonic day 10.5
(more )
Specimen
6C:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: somite 11 dermomyotome |
Strong |
Regionally restricted |
6C
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Expression is strong in the dorsal part, weak in the ventral part.
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| TS17: somite 12 dermomyotome |
Strong |
Regionally restricted |
6C
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Expression is strong in the dorsal part, weak in the ventral part.
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| TS17: trunk myotome |
Strong |
Regionally restricted |
6C
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Expression is strong in the dorsal part, weak in the ventral part. Expression is in all but the most caudal 4-5 somites.
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Specimen
7:
embryonic day 9.5
(more )
Specimen
7:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: dermatome |
Present |
Regionally restricted |
7_MRF4
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Expression is in the myotome.
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Specimen
8A:
embryonic day 9.5
(more )
Note: Double labeled with MHC (fluorescence).
Specimen
8A:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled with MHC (fluorescence).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: myotome |
Present |
Regionally restricted |
8A
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Expression is in myoblasts in the dorsal region of the myotome.
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| TS15: somite 13 dermomyotome |
Present |
Regionally restricted |
8A
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Expression is in myoblasts in the dorsal region of the myotome.
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| TS15: somite 14 dermomyotome |
Present |
Regionally restricted |
8A
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Expression is in myoblasts in the dorsal region of the myotome.
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Specimen
not shown E8.5:
embryonic day 8.5
(more )
Specimen
not shown E8.5:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
not shown E9:
embryonic day 9.0
(more )
Note: 13-19 somite stage embryo.
Specimen
not shown E9:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 9.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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13-19 somite stage embryo.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS14: somite |
Present |
Regionally restricted |
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Expression is in the most rostral somites. The caudal limit in a 16 somite embryo is in the 3-4 most rostral somites.
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Specimen
not shown E10.5:
embryonic day 10.5
(more )
Specimen
not shown E10.5:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: tail dermomyotome |
Present |
Regionally restricted |
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Expression is in the dorsal myotome.
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Analysis Tools