Gene Expression Data
Assay Details
Assay
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Reference:
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J:20949
Smith TH, et al., Somite subdomains, muscle cell origins, and the four muscle regulatory factor proteins. J Cell Biol. 1994 Oct;127(1):95-105
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6117033
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Gene symbol:
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Myf5
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Gene name:
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myogenic factor 5
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Antibody:
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Myf-5 antibody
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
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Results
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Specimen
1A:
embryonic day 9.0
(more )
Note: 13 somite stage embryo.
Specimen
1A:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 9.0
|
Sex:
|
Not Specified
|
Type:
|
section
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Fixation:
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4% Paraformaldehyde
|
Embedding:
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Cryosection
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Note:
|
13 somite stage embryo.
|
Specimen
1B:
embryonic day 9.0
(more )
Note: 13 somite stage embryo.
Specimen
1B:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 9.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
13 somite stage embryo.
|
Structure |
Level |
Pattern |
Image |
Note |
TS14: somite 04 |
Present |
Regionally restricted |
1B
|
Expression is in the most dorsal portion near the neural tube.
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TS14: somite 05 |
Present |
Regionally restricted |
1B
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Expression is in the most dorsal portion near the neural tube.
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Specimen
1C:
embryonic day 9.5
(more )
Note: 24 somite stage embryo.
Specimen
1C:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 9.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
24 somite stage embryo.
|
Structure |
Level |
Pattern |
Image |
Note |
TS15: somite 16 |
Present |
Regionally restricted |
1C
|
Expression is in the most dorsal portion near the neural tube.
|
TS15: somite 17 |
Present |
Regionally restricted |
1C
|
Expression is in the most dorsal portion near the neural tube.
|
Specimen
2A:
embryonic day 9.0
(more )
Note: 13 somite stage embryo.
Specimen
2A:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 9.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
13 somite stage embryo.
|
Structure |
Level |
Pattern |
Image |
Note |
TS14: somite 04 |
Present |
Regionally restricted |
2A
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Expression is in the anterior portion of the somites.
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TS14: somite 05 |
Present |
Regionally restricted |
2A
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Expression is in the anterior portion of the somites.
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TS14: somite 06 |
Present |
Regionally restricted |
2A
|
Expression is in the anterior portion of the somites.
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Specimen
3A:
embryonic day 9.5
(more )
Note: 24-26 somite stage embryo.
Specimen
3A:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 9.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
24-26 somite stage embryo.
|
Specimen
5A:
embryonic day 9.5
(more )
Specimen
5A:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 9.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
5C:
embryonic day 9.5
(more )
Specimen
5C:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 9.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS15: somite 09 |
Present |
Regionally restricted |
5C
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Expression is dorsal.
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TS15: somite 10 |
Present |
Regionally restricted |
5C
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Expression is dorsal.
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Specimen
5D:
embryonic day 9.5
(more )
Specimen
5D:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 9.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS15: somite 11 |
Present |
Regionally restricted |
5D
|
Expression is dorsal.
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TS15: somite 12 |
Present |
Regionally restricted |
5D
|
Expression is dorsal.
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Specimen
5F:
embryonic day 9.5
(more )
Specimen
5F:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 9.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS15: somite 11 dermomyotome |
Present |
Regionally restricted |
5F
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Expression is in the dorsal-medial lip of the dermatome near the neural tube.
|
TS15: somite 12 dermomyotome |
Present |
Regionally restricted |
5F
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Expression is in the dorsal-medial lip of the dermatome near the neural tube.
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Specimen
6A:
embryonic day 10.5
(more )
Specimen
6A:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 10.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
7:
embryonic day 9.5
(more )
Specimen
7:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 9.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS15: trunk dermatome |
Present |
Regionally restricted |
7_Myf-5
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Expression is in the dorsal, posterior, and anterior lips of the dermatome.
|
Specimen
not shown E8.5:
embryonic day 8.5
(more )
Specimen
not shown E8.5:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 8.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
not shown E9:
embryonic day 9.0
(more )
Note: 13-19 somite stage embryo.
Specimen
not shown E9:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 9.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
13-19 somite stage embryo.
|
Structure |
Level |
Pattern |
Image |
Note |
TS14: somite |
Present |
Regionally restricted |
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Expression is in the most rostral somites. The caudal limit in a 16 somite embryo is in the 9-10 most rostral somites.
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