Probe:	Hop probe2
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS20: cerebral cortex	Present	Regionally restricted	1C	Expression first detectable in forebrain in most medial aspect of cortex (anlage of archipallium).
TS20: heart	Present		1C
TS20: spinal cord ventricular layer	Present		1C

Structure	Level	Pattern	Image	Note
TS20: basal columns	Present		1D	Higher expression in basal plate than in alar plate.
TS20: alar columns	Ambiguous		1D	Higher expression in basal plate than in alar plate.
TS20: spinal cord floor plate	Absent		1D
TS20: spinal cord roof plate	Absent		1D
TS20: hindbrain	Present	Regionally restricted	1D	Expression in anlage of cochleo-vestibular hindbrain nucleus.

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex	Present	Regionally restricted	1E	Expression persists in medial cortex.

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex	Present	Regionally restricted	1F	Expression persists in medial cortex.

Structure	Level	Pattern	Image	Note
TS22: forebrain	Weak		1G	Authors state forebrain expression levels remains low at E14.5

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex ventricular layer	Present	Graded	1H	Gradient of expression in cortical ventricular zone, with high expression rostro-medially and low expression caudo-laterally

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex	Present	Regionally restricted	1I inset , 1I	Expression in cortical hem.
TS24: hippocampal formation	Present	Regionally restricted	1I inset , 1I	Expression in a stream of cells leaving the dentate notch towards the developing dentate gyrus.

Structure	Level	Pattern	Image	Note
TS24: hippocampal formation	Present	Regionally restricted	1J	Expression in dentate gyrus.

Structure	Level	Pattern	Image	Note
TS26: forebrain	Strong	Regionally restricted	1K	Peak expression in forebrain.
TS26: cerebral cortex ventricular zone	Strong	Regionally restricted	1K	Strong expression in cortical ventricular zone forming a medial-high to lateral-low gradient.

Structure	Level	Pattern	Image	Note
TS26: cerebral cortex	Present	Regionally restricted	1L , 1L inset	Expression in cortical hem.
TS26: dentate gyrus	Present		1L , 1L inset

Structure	Level	Pattern	Image	Note
TS26: dentate gyrus	Present		1M	Expression in dentate gyrus with more expressing cells having reached the dentate gyrus.

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus	Present		1N	Expression in entire dentate gyrus and CA3 field of hippocampus.
TS28: hippocampus CA3	Present		1N	Expression in entire dentate gyrus and CA3 field of hippocampus.

Structure	Level	Pattern	Image	Note
TS28: ependyma	Present		1O	Expression in ependymal cell layer.

Structure	Level	Pattern	Image	Note
TS28: cerebellum	Present	Regionally restricted	1P	In cerebellum, expression in cells most likely representing a subpopulation of Bergmann glia cells.

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus	Present	Regionally restricted	1Q	Expression in subgranular layer of dentate gyrus in cells resembling the dentate gyrus stem cell population.

Structure	Level	Pattern	Image	Note
TS28: cerebellum	Present	Regionally restricted	1R	In cerebellum, expression in Bergmann glia cells.